An antimicrobial peptide is downregulated in the small intestine of Eimeria maxima-infected chickens
| Autor: | Derek A. Emmerson, A. P. McElroy, Eric A. Wong, S. Casterlow, H. Li, Elizabeth R. Gilbert, Rami A. Dalloul |
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| Rok vydání: | 2011 |
| Předmět: |
animal structures
Eimeria Microbiology Jejunum Lesion Gene expression Intestine Small medicine Animals Genetic Predisposition to Disease Poultry Diseases Oligonucleotide Array Sequence Analysis Innate immune system biology Coccidiosis Gene Expression Profiling General Medicine biology.organism_classification medicine.disease Small intestine medicine.anatomical_structure Eimeria maxima Gene Expression Regulation embryonic structures Animal Science and Zoology medicine.symptom Chickens Antimicrobial Cationic Peptides |
| Zdroj: | Poultry science. 90(6) |
| ISSN: | 0032-5791 |
| Popis: | Avian coccidiosis is a major disease of poultry caused by the intestinal protozoa Eimeria. Infection leads to reduced feed efficiency and BW gain, resulting in severe economic losses for the poultry industry. Aviagen line A and line B birds show a differential response to Eimeria infection, with line B birds exhibiting higher lesion scores and mortality. The objective of this study was to examine differential intestinal gene expression between 2-wk-old line A and B chicks in response to a challenge with Eimeria maxima. After challenge with 1 × 104 oocysts/chick, more than 40% of line A chicks had lesion scores of 0 to 1 (scale of 0 to 4), similar to control chicks. In contrast, all line B chicks challenged at this same dose had lesion scores of 2 to 4. Total RNA was extracted from the jejunum of control and challenged chicks from both lines A and B. Microarray analysis revealed that liver-expressed antimicrobial peptide 2 (LEAP-2), a component of the innate immune system, was downregulated 20-fold in line A challenged chicks with lesion scores of 2 to 4 compared with line A control chicks, and was downregulated 11- to 71-fold in line B challenged chicks with lesion scores of 2 to 4 compared with line B control chicks. Liver-expressed antimicrobial peptide 2 was downregulated less than 2-fold in line A challenged chicks with lesion scores of 1 compared with line A control chicks, indicating that these chicks were similar to control chicks in their expression level of LEAP-2. Other genes (cytochrome P450, heat shock protein 25, keratin 19, and amino acid transporter ASCT1) showed different patterns of over- or underexpression. The expression of LEAP-2 was verified using real-time PCR, revealing a correlation between lesion score and magnitude of LEAP-2 downregulation for both line A and line B chicks. Thus, LEAP-2 may serve as a useful marker for identification of chickens resistant to E. maxima infection and potentially other Eimeria spp. |
| Databáze: | OpenAIRE |
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