The support-on-support concept for in-situ oligonucleotide synthesis on nanoparticles

Autor:	Shlomo Margel, M. Koch, P. Lapido, Michael Hinz, R. Ditz, Hartmut Seliger
Rok vydání:	2007
Předmět:	In situ Oligonucleotide Composite number Nanoparticle Metal Nanoparticles Nanotechnology Dextrans General Medicine Oligonucleotide synthesis Biochemistry Ferrosoferric Oxide chemistry.chemical_compound chemistry Oligodeoxyribonucleotides Genetics Molecular Medicine Nanoparticles Polystyrenes Spectrophotometry Ultraviolet Polystyrene Layer (electronics) Dissolution Polyhydroxyethyl Methacrylate
Zdroj:	Nucleosides, nucleotidesnucleic acids. 26(8-9)
ISSN:	1525-7770
Popis:	Oligonucleotide-loaded nanoparticles, which are of interest for biomedical application, up to now, could not be prepared by in-situ synthesis, due to difficulty of handling in automated synthesizers. To overcome this problem, we have introduced the “support-on-support” concept. It is based on the reversible anchoring of nanoparticles to the surface of microparticles. These composite beads easily can be used for automated synthesis, being released after completion of chain elongations. As examples, dextran-coated magnetite nanoparticles were attached to polystyrene microparticles through (1) a gelatine or (2) a silica layer. Release involved dissolution of the bonding layer by (1) proteases or (2) alkali.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2df84c7a47ea525516a5c8ac4e8824a6 https://pubmed.ncbi.nlm.nih.gov/18058558 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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