Monoclonal Antibodies to Shigella Lipopolysaccharide Are Useful for Vaccine Production
Autor: | Heather Wenzel, Mark A. Smith, Jisheng Lin, Moon H. Nahm, Robert W. Kaminski, William H. Benjamin |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Microbiology (medical) Vaccine evaluation medicine.drug_class 030106 microbiology Clinical Biochemistry Immunology Shigella sonnei Biology medicine.disease_cause Monoclonal antibody Shigella flexneri Microbiology 03 medical and health sciences Shigella Vaccines medicine Animals Immunology and Allergy Shigella Shigella vaccine Immunoassay Mice Inbred BALB C Vaccines medicine.diagnostic_test Antibodies Monoclonal O Antigens biology.organism_classification Antibodies Bacterial 030104 developmental biology Vaccine Potency Female |
Zdroj: | Clinical and Vaccine Immunology. 23:681-688 |
ISSN: | 1556-679X 1556-6811 |
Popis: | There is a significant need for an effective multivalent Shigella vaccine that targets the most prevalent serotypes. Most Shigella vaccines under development utilize serotype-specific lipopolysaccharides (LPSs) as a major component based on protection and epidemiological data. As vaccine formulations advance from monovalent to multivalent, assays and reagents need to be developed to accurately and reproducibly quantitate the amount of LPSs from multiple serotypes in the final product. To facilitate this effort, we produced 36 hybridomas that secrete monoclonal antibodies (MAbs) against the O antigen on the LPS from Shigella flexneri 2a, Shigella flexneri 3a, and Shigella sonnei . We used six of these monoclonal antibodies for an inhibition enzyme-linked immunosorbent assay (iELISA), measuring LPSs with high sensitivity and specificity. It was also demonstrated that the Shigella serotype-specific MAbs were useful for bacterial surface staining detected by flow cytometry. These MAbs are also useful for standardizing the serum bactericidal assay (SBA) for Shigella . Functional assays, such as the in vitro bactericidal assay, are necessary for vaccine evaluation and may serve as immunological correlates of immunity. An S. flexneri 2a-specific monoclonal antibody killed S. flexneri 2b isolates, suggesting that S. flexneri 2a LPS may induce cross-protection against S. flexneri 2b. Overall, the Shigella LPS-specific MAbs described have potential utility to the vaccine development community for assessing multivalent vaccine composition and as a reliable control for multiple immunoassays used to assess vaccine potency. |
Databáze: | OpenAIRE |
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