Biocontrol of Biofilm Formation: Jamming of Sessile-Associated Rhizobial Communication by Rhodococcal Quorum-Quenching

Autor: Linossier, I, Bourigault, Yvann, Rodrigues, Sophie, Crépin, Alexandre, Chane, Andrea, Taupin, Laure, Bouteiller, Mathilde, Dupont, Charly, Merieau, Annabelle, Konto-Ghiorghi, Yoan, Boukerb, Amine, turner, Marie, Hamon, Céline, Dufour, Alain, Barbey, Corinne, Latour, Xavier
Přispěvatelé: Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2021
Předmět:
hairy root
quorum-quenching
lifestyle switch
N-acyl homoserine lactones
Rhizobium (Agrobacterium) rhizogenes
[SDV]Life Sciences [q-bio]
Agrobacterium
biological control
Acyl-Butyrolactones
biofilm
Lactonase
Rhodococcus
Biology (General)
Spectroscopy
0303 health sciences
biology
communication
Chemistry
Quorum Sensing
food and beverages
General Medicine
Computer Science Applications
Quorum Quenching
Rhizobium
QH301-705.5
quorum-sensing
Article
Catalysis
Microbiology
Inorganic Chemistry
03 medical and health sciences
Bacterial Proteins
Physical and Theoretical Chemistry
Rhodococcus erythropolis
QD1-999
Molecular Biology
030304 developmental biology
030306 microbiology
Organic Chemistry
Biofilm
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Rhizobium rhizogenes
Quorum sensing
Biofilms
biology.protein
bacteria
Carboxylic Ester Hydrolases
Bacteria
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, MDPI, 2021, 22 (15), pp.8241. ⟨10.3390/ijms22158241⟩
International Journal of Molecular Sciences, Vol 22, Iss 8241, p 8241 (2021)
International Journal of Molecular Sciences, 2021, 22 (15), pp.8241. ⟨10.3390/ijms22158241⟩
Volume 22
Issue 15
ISSN: 1422-0067
1661-6596
Popis: Biofilms are complex structures formed by a community of microbes adhering to a surface and/or to each other through the secretion of an adhesive and protective matrix. The establishment of these structures requires a coordination of action between microorganisms through powerful communication systems such as quorum-sensing. Therefore, auxiliary bacteria capable of interfering with these means of communication could be used to prevent biofilm formation and development. The phytopathogen Rhizobium rhizogenes, which causes hairy root disease and forms large biofilms in hydroponic crops, and the biocontrol agent Rhodococcus erythropolis R138 were used for this study. Changes in biofilm biovolume and structure, as well as interactions between rhizobia and rhodococci, were monitored by confocal laser scanning microscopy with appropriate fluorescent biosensors. We obtained direct visual evidence of an exchange of signals between rhizobia and the jamming of this communication by Rhodococcus within the biofilm. Signaling molecules were characterized as long chain (C14) N-acyl-homoserine lactones. The role of the Qsd quorum-quenching pathway in biofilm alteration was confirmed with an R. erythropolis mutant unable to produce the QsdA lactonase, and by expression of the qsdA gene in a heterologous host, Escherichia coli. Finally, Rhizobium biofilm formation was similarly inhibited by a purified extract of QsdA enzyme.
Databáze: OpenAIRE
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