Knockdown of the NHR-8 nuclear receptor enhanced sensitivity to the lipid-reducing activity of alkaloids in Caenorhabditis elegans
| Autor: | Fumihiko Sato, Yuriko Kawasaki, Yit-Lai Chow |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
detoxification response
Berberine Receptors Cytoplasmic and Nuclear Biology Pharmacology Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry nuclear hormone receptor-8 (NHR-8) chemistry.chemical_compound RNA interference parasitic diseases Animals RNA Small Interfering Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Benzophenanthridines Gene knockdown Plant Extracts Organic Chemistry isoquinoline alkaloid Wild type Biological activity General Medicine biology.organism_classification Isoquinolines Lipid Metabolism High-Throughput Screening Assays bioactivity screening Nuclear receptor chemistry Gene Expression Regulation Inactivation Metabolic Xenobiotic Rhizome Biotechnology Coptis Signal Transduction |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 78(12) |
| ISSN: | 1347-6947 |
| Popis: | Caenorhabditis elegans is a versatile, whole-organism model for bioactivity screening. However, this worm has extensive defensive mechanisms against xenobiotics which limit its use for screening of pharmacologically active compounds. In this study, we report that knockdown of nhr-8, a gene involved in the xenobiotic response, increased the worm’s sensitivity to the lipid-reducing effects of some isoquinoline alkaloids, especially berberine. On the other hand, crude extract of rhizome and cultured cells showed enhanced biological activity compared to the pure alkaloids in wild type worm, but this enhanced activity was not detected in nhr-8 RNAi worm, suggesting that some components in cell extracts might interfere with the defense response in this worm. The possibility of using C. elegans as a model for screening bioactive chemicals is discussed. |
| Databáze: | OpenAIRE |
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