Differential Gene Expression in Peripheral White Blood Cells with Permissive Underfeeding and Standard Feeding in Critically Ill Patients: A Descriptive Sub-study of the PermiT Randomized Controlled Trial
| Autor: | Yaseen M. Arabi, Ibrahim Al Abdulkareem, Mohammed AlBalwi, Hani Tamim, Ali H. Hajeer, Haitham Alkadi, Musharaf Sadat, Walid Almashaqbeh, Hasan M. Al-Dorzi, Dunia Jawdat, Abdulaziz Al-Dawood, Lara Afesh, Deemah Alwadaani, G. K. UdayaRaja |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Critical Care Critical Illness lcsh:Medicine Physiology Buffy coat Enteral administration Article law.invention Transcriptome Young Adult 03 medical and health sciences 0302 clinical medicine Randomized controlled trial law Gene expression Leukocytes Humans Medicine lcsh:Science Gene Aged Caloric Restriction Retrospective Studies Multidisciplinary business.industry Microarray analysis techniques Gene Expression Profiling Malnutrition lcsh:R Standard of Care 030208 emergency & critical care medicine Middle Aged Gene expression profiling 030104 developmental biology Female lcsh:Q Nutrition Therapy Energy Intake business |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-8 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The effect of short-term caloric restriction on gene expression in critically ill patients has not been studied. In this sub-study of the PermiT trial (Permissive Underfeeding or Standard Enteral Feeding in Critically Ill Adults Trial- ISRCTN68144998), we examined gene expression patterns in peripheral white blood cells (buffy coat) associated with moderate caloric restriction (permissive underfeeding) in critically ill patients compared to standard feeding. Blood samples collected on study day 1 and 14 were subjected to total RNA extraction and gene expression using microarray analysis. We enrolled 50 patients, 25 in each group. Among 1751 tested genes, 332 genes in 12 pathways were found to be significantly upregulated or downregulated between study day 1 and 14 (global p value for the pathway ≤ 0.05). Using the heatmap, the differential expression of genes from day 1 to 14 in the permissive underfeeding group was compared to the standard feeding group. We further compared gene expression signal intensity in permissive underfeeding compared standard feeding by constructing univariate and multivariate linear regression models on individual patient data. We found differential expression of several genes with permissive underfeeding, most notably those related to metabolism, autophagy and other cellular functions, indicating that moderate differences in caloric intake trigger different cellular pathways. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|