A novel method for simple detection of mutations conferring drug resistance in Mycobacterium leprae, based on a DNA microarray, and its applicability in developing countries
| Autor: | Chie Nakajima, Paul Saunderson, Kyaw Kyaw, Ma. Victoria F. Balagon, Yasuhiko Suzuki, Masanao Makino, Khin Saw Aye, Masanori Matsuoka, Robert H. Gelber, Esterlina V. Tan |
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| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Philippines Leprostatic Agents Myanmar Drug resistance medicine.disease_cause Microbiology Genome Bacterial Proteins Leprosy Drug Resistance Bacterial Prevalence medicine Humans Developing Countries Mycobacterium leprae Oligonucleotide Array Sequence Analysis Genetics Mutation biology Gene Expression Regulation Bacterial General Medicine biology.organism_classification medicine.disease rpoB DNA microarray Rifampicin medicine.drug |
| Zdroj: | Journal of Medical Microbiology. 57:1213-1219 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.2008/002600-0 |
| Popis: | A simple method to detect mutations in the genome of Mycobacterium leprae that confer resistance to key drugs for leprosy was exploited on the basis of a reverse hybridization system. A series of oligonucleotide probes corresponding to each mutation in the folP1, rpoB and gyrA genes for dapsone, rifampicin and ofloxacin resistance, respectively, were selected and fixed on a glass slide as capture probes, to develop a DNA microarray termed the leprosy drug susceptibility-DNA microarray (LDS-DA). Mutations in clinical isolates of M. leprae were successfully identified by the LDS-DA. Feasibility studies were conducted to evaluate the performance of the LDS-DA in two developing countries, Myanmar and the Philippines. The high concordance of the results obtained by this method with the results of nucleotide sequencing strongly supports the applicability of the LDS-DA as a drug susceptibility test in place of sequencing, a time-consuming and costly procedure. This is a rapid and simple method for the simultaneous susceptibility testing of three front-line drugs for leprosy, and solves the problems of previously reported methods. |
| Databáze: | OpenAIRE |
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