PAL31 may play an important role as inflammatory modulator in the repair process of the spinal cord injury rat
| Autor: | Kin-Fu Chak, Huai Sheng Kuo, Henrich Cheng, Ming Chu Tsai, Li Fen Shen |
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| Rok vydání: | 2009 |
| Předmět: |
Small interfering RNA
Nitric Oxide Synthase Type II Inflammation Apoptosis Cell Cycle Proteins Nerve Tissue Proteins Biology CD8-Positive T-Lymphocytes Biochemistry Rats Sprague-Dawley Cellular and Molecular Neuroscience medicine In Situ Nick-End Labeling Animals Electrophoresis Gel Two-Dimensional Spinal cord injury Chemokine CCL2 Spinal Cord Injuries Cell Line Transformed Activator (genetics) Kinase Caspase 3 Macrophages Nuclear Proteins Recovery of Function Ectodysplasins medicine.disease Cyclic AMP-Dependent Protein Kinases Cell biology Rats Nitric oxide synthase Disease Models Animal STAT1 Transcription Factor Gene Expression Regulation Spinal Cord Cell culture Immunology biology.protein Cytokines Fibroblast Growth Factor 1 Female medicine.symptom Molecular Chaperones |
| Zdroj: | Journal of neurochemistry. 108(5) |
| ISSN: | 1471-4159 |
| Popis: | Functional regeneration in a complete T8 transection model Cheng et al. (1996) and most recently, acidic fibroblast growth factor (aFGF; also known as FGF-1) involved in the repair process of the spinal cord injury (SCI) rat Tsai et al. (2008) have been reported. To further reveal the mechanism of the repair process of SCI, in additionally, we have identified a 30 kDa specific protein kinase A substrate induced at 6 days after SCI. However, the induction of the transducing signal was reduced in samples treated with aFGF. The 30 kDa protein was purified and identified by mass spectrometry as a novel protein, PAL31. The results of immunohistochemical study showed that PAL31 is abundantly expressed in the epicenter of the injured spinal cord and colocalizes with ED1-positive cells (macrophages) and CD8 T lymphocytes. Over-expression of PAL31 in RAW 264.7 cells resulted in the down-regulation of macrophage chemoattractant protein 1, inducible nitric oxide synthase, and signal transducer and activator of transcription-1. However, knockdown of PAL31 by small interfering RNA seems to lead to apoptosis when the cells were treated with inflammatory inducers. These experimental results suggest that PAL31 may involve in the modulation of the inflammatory response and, at the same time, prevent apoptosis process of macrophage after SCI. |
| Databáze: | OpenAIRE |
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