Metabolic engineering of Amycolatopsis japonicum for optimized production of [S,S]-EDDS, a biodegradable chelator
| Autor: | Jernej Smole, Eva Doskocil, Martin Roth, Michael Biermann, Wolfgang Wohlleben, Evi Stegmann, Gregor Kosec, Simone Edenhart, Martin Kavšček, Marius Spohn, Marius Denneler, Bettina Bardl, Tadeja Amon |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
chemistry.chemical_element Bioengineering Zinc 01 natural sciences Applied Microbiology and Biotechnology Chemical synthesis Metabolic engineering 03 medical and health sciences chemistry.chemical_compound Bioreactors EDDS 010608 biotechnology Escherichia coli Soil Pollutants Organic chemistry Amycolatopsis japonicum Chelation Promoter Regions Genetic Amycolatopsis Edetic Acid Chelating Agents 030304 developmental biology 0303 health sciences Aqueous solution Ethylenediamines body regions Biodegradation Environmental Metabolic Engineering chemistry Fermentation Biotechnology |
| Zdroj: | Metabolic Engineering. 60:148-156 |
| ISSN: | 1096-7176 |
| DOI: | 10.1016/j.ymben.2020.04.003 |
| Popis: | The actinomycete Amycolatopsis japonicum is the producer of the chelating compound [S,S]-ethylenediamine-disuccinc acid (EDDS). [S,S]-EDDS is an isomer of ethylenediamine-tetraacetic acid (EDTA), an economically important chelating compound that suffers from an extremely poor degradability. Frequent use of the persistent EDTA in various industrial and domestic applications has caused an accumulation of EDTA in soil as well as in aqueous environments. As a consequence, EDTA is the highest concentrated anthropogenic compound present in water reservoirs. The [S,S]-form of EDDS has chelating properties similar to EDTA, however, in contrast to EDTA it is readily biodegradable. In order to compete with the cost-effective chemical synthesis of EDTA, we aimed to optimize the biotechnological production of [S,S]-EDDS in A. japonicum by using metabolic engineering approaches. Firstly, we integrated several copies of the [S,S]-EDDS biosynthetic genes into the chromosome of A. japonicum and replaced the native zinc responsive promoter with the strong synthetic constitutive promoter SP44*. Secondly, we increased the supply of O-phospho-serine, the direct precursor of [S,S]-EDDS. The combination of these approaches together with the optimized fermentation process led to a significant improvement in [S,S]-EDDS up to 9.8 g/L with a production rate of 4.3 mg/h/g DCW. |
| Databáze: | OpenAIRE |
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