Catalase catalyzes nitrotyrosine formation from sodium azide and hydrogen peroxide
| Autor: | Hiroyuki Nakamura, Norio Kodama, Keiki Ogino, Madoka Nakajima, Takeshi Maekawa, Hirohumi Nagase, Akihiro Yamada, Daikai Sadamitsu |
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| Rok vydání: | 2001 |
| Předmět: |
inorganic chemicals
Time Factors Photochemistry Biochemistry Medicinal chemistry Catalysis Superoxide dismutase chemistry.chemical_compound Nitration Animals Nitrite Sodium Azide Hydrogen peroxide Nitrites Nitrates biology Nitrotyrosine Serum Albumin Bovine Hydrogen Peroxide General Medicine Hydrogen-Ion Concentration Catalase chemistry Luminescent Measurements biology.protein Tyrosine Sodium azide Cattle Peroxynitrite |
| Zdroj: | Free Radical Research. 35:735-747 |
| ISSN: | 1029-2470 1071-5762 |
| DOI: | 10.1080/10715760100301241 |
| Popis: | Sodium azide (NaN3) is known as an inhibitor of catalase, and a nitric oxide (NO) donor in the presence of catalase and H2O2. We showed here that catalase-catalyzed oxidation of NaN3 can generate reactive nitrogen species which contribute to tyrosine nitration in the presence of H2O2. The formation of free-tyrosine nitration and protein-bound tyrosine nitration by the NaN3/catalase/H2O2 system showed a maximum level at pH 6.0. Free-tyrosine nitration induced by peroxynitrite was inhibited by ethanol and dimethylsulfoxide (DMSO), and augmented by superoxide dismutase (SOD). However, free-tyrosine nitration induced by the NaN3/catalase/H2O2 system was not affected by ethanol, DMSO and SOD. NO2- and NO donating agents did not affect free-tyrosine nitration by the NaN3/catalase/H2O2 system. The reaction of NaN3 with hydroxyl radical generating system showed free-tyrosine nitration, but no formation of nitrite and nitrate. The generation of nitrite (NO2-) and nitrate (NO3-) by the NaN3/catalase/H2O2 system was maximal at pH 5.0. These results suggested that the oxidation of NaN3 by the catalase/H2O2 system generates unknown peroxynitrite-like reactive nitrogen intermediates, which contribute to tyrosine nitration. |
| Databáze: | OpenAIRE |
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