Contrasting genetic diversity and structure among Malagasy Ralstonia pseudosolanacearum phylotype I populations inferred from an optimized Multilocus Variable Number of Tandem Repeat Analysis scheme
| Autor: | Philippe Prior, Adrien Rieux, Gilles Cellier, Niry Tiana Dianzinga, Emeline Rebert, Laurent Costet, Hasina Rasoamanana, Fabien Guérin, Miharisoa-Mirana Gauche, Noura Yahiaoui, Yann Pecrix, Isabelle Robène, Stéphane Poussier, Santatra Ravelomanantsoa |
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| Přispěvatelé: | Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de La Réunion (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre National de Recherche Appliquée au Développement Rural (FOFIFA), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Laboratoire de la Santé des Végétaux, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Unité Ravageurs et agents pathogènes tropicaux, This project was supported by Agropolis Fondation under the reference ID 1403-011 through the 'Investissements d'avenir' program (Labex Agro: ANR-10-LABX-0001). This work was funded by the European Union: European regional development fund (ERDF, programme INTERREG V), by the Conseil Regional de La Reunion and by the Centre de Cooperation Internationale en Recherche agronomique pour le Developpement (CIRAD). HR and SR received doctoral fellowships from the Conseil Regional de La Reunion. NY was supported by a doctoral fellowship from ANSES (Agence nationale de securite sanitaire de l'alimentation, de l'environnement et du travail) and CIRAD, ANR-10-LABX-0001,AGRO,Agricultural Sciences for sustainable Development(2010), Laboratoire de la santé des végétaux (LSV), Unité Ravageurs et agents pathogènes tropicaux (RAPT), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Heredity
Epidemiology [SDV]Life Sciences [q-bio] Artificial Gene Amplification and Extension Ralstonia Polymerase Chain Reaction F30 - Génétique et amélioration des plantes Geographical Locations Génétique des populations Medicine and Health Sciences Phylogeny 2. Zero hunger Phylotype 0303 health sciences Ralstonia solanacearum Multidisciplinary biology Ecology food and beverages Genomics Bacterial Typing Techniques Épidémiologie Variable number tandem repeat Genetic Mapping PCR Tandem Repeat Sequences Genetic Epidemiology Medicine Research Article Species complex Flétrissement Genotype Ecological Metrics Science Multiple Loci VNTR Analysis Research and Analysis Methods 03 medical and health sciences Variation génétique Genetics Madagascar Molecular Biology Techniques Molecular Biology 030304 developmental biology H20 - Maladies des plantes Bactérie pathogène Genetic diversity Evolutionary Biology Molecular epidemiology Population Biology 030306 microbiology Ecology and Environmental Sciences Genetic Variation Biology and Life Sciences Species Diversity 15. Life on land biology.organism_classification Maladie bactérienne Haplotypes Evolutionary biology Genetic Loci People and Places Africa Multilocus sequence typing Population Genetics Multilocus Sequence Typing |
| Zdroj: | PloS One PLoS ONE PLoS ONE, Public Library of Science, 2020, 15 (12), pp.e0242846. ⟨10.1371/journal.pone.0242846⟩ PLoS ONE, Vol 15, Iss 12, p e0242846 (2020) PLoS ONE, 2020, 15 (12), pp.e0242846. ⟨10.1371/journal.pone.0242846⟩ |
| ISSN: | 1932-6203 |
| Popis: | TheRalstonia solanacearumspecies complex (RSSC), composed of three species and four phylotypes, are globally distributed soil-borne bacteria with a very broad host range. In 2009, a devastating potato bacterial wilt outbreak was declared in the central highlands of Madagascar, which reduced the production of vegetable crops including potato, eggplant, tomato and pepper. A molecular epidemiology study of Malagasy RSSC strains carried out between 2013 and 2017 identifiedR.pseudosolanacearum(phylotypes I and III) andR.solanacearum(phylotype II). A previously published population biology analysis of phylotypes II and III using two MultiLocus Variable Number of Tandem Repeats Analysis (MLVA) schemes revealed an emergent epidemic phylotype II (sequevar 1) group and endemic phylotype III isolates. We developed an optimized MLVA scheme (RS1-MLVA14) to characterize phylotype I strains in Madagascar to understand their genetic diversity and structure. The collection included isolates from 16 fields of different Solanaceae species sampled in Analamanga and Itasy regions (highlands) in 2013 (123 strains) and in Atsinanana region (lowlands) in 2006 (25 strains). Thirty-one haplotypes were identified, two of them being particularly prevalent: MT007 (30.14%) and MT004 (16.44%) (sequevar 18). Genetic diversity analysis revealed a significant contrasting level of diversity according to elevation and sampling region. More diverse at low altitude than at high altitude, the Malagasy phylotype I isolates were structured in two clusters, probably resulting from different historical introductions. Interestingly, the most prevalent Malagasy phylotype I isolates were genetically distant from regional and worldwide isolates. In this work, we demonstrated that the RS1-MLVA14 scheme can resolve differences from regional to field scales and is thus suited for deciphering the epidemiology of phylotype I populations. |
| Databáze: | OpenAIRE |
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