Lower cytokine secretion ex vivo by natural killer T cells in HIV-infected individuals is associated with higher CD161 expression
| Autor: | Johan K. Sandberg, Steven G. Deeks, Michael G. Rosenberg, Esper G. Kallas, Jennifer E. Snyder-Cappione, Douglas F. Nixon, Carlotta Kuylenstierna, Frederick Hecht, Karina I. Carvalho, Christopher P. Loo |
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| Rok vydání: | 2009 |
| Předmět: |
Time Factors
medicine.medical_treatment Immunology Galactosylceramides HIV Infections chemical and pharmacologic phenomena Peripheral blood mononuclear cell Natural killer cell Interferon-gamma Immune system T-Lymphocyte Subsets Immune Tolerance medicine Humans Immunology and Allergy Cells Cultured biology Tumor Necrosis Factor-alpha hemic and immune systems Th1 Cells Natural killer T cell Infectious Diseases Cytokine medicine.anatomical_structure CD1D biology.protein Cytokines Natural Killer T-Cells Cytokine secretion Tumor necrosis factor alpha Antigens CD1d NK Cell Lectin-Like Receptor Subfamily B |
| Zdroj: | AIDS. 23:1965-1970 |
| ISSN: | 0269-9370 |
| DOI: | 10.1097/qad.0b013e32832b5134 |
| Popis: | Objective: Natural killer T (NKT) cells are efficiently targeted by HIV and severely reduced in numbers in the circulation of infected individuals. The functional capacityof the remaining NKT cells in HIV-infected individuals is poorly characterized. This study measured NKT cell cytokine production directly ex vivo and compared these responses with both the disease status and NKT subset distribution of individual patients. Methods: NKT cell frequencies, subsets, and ex-vivo effector functions were measured in the peripheral blood mononuclear cells of HIV-infected patients and healthy controls by flow cytometry. We measured cytokines from NKT cells after stimulation with either a-galactosyl ceramide-loaded CD1d dimers (DimerX-aGalCer) or phorbol myristate acetate and ionomycin. Results: The frequencies of NKT cells secreting interferon-g and tumor necrosis factora were significantly lower in HIV-infected patients than healthy controls after DimerXaGalCer treatment, but responses were similar after treatment with phorbol myristate acetate and ionomycin.The magnitude of the interferon-gresponse to DimerX-aGalCer correlated inversely with the number of years of infection. Both interferon-g and tumor necrosis factor-a production in response to DimerX-aGalCer correlated inversely with CD161 expression. Conclusion: The ex-vivo Th1 responses of circulating NKT cells to CD1d-glycolipid complexes are impaired in HIV-infected patients. NKT cell functions may be progressively lost over time in HIV infection, and CD161 is implicated in the regulation of NKT cell responsiveness. 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins AIDS 2009, 23:1965‐1970 |
| Databáze: | OpenAIRE |
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