Arabidopsis tRNA-derived fragments as potential modulators of translation
| Autor: | Stéphanie Lalande, Thalia Salinas-Giegé, Laurence Drouard, Rémy Merret |
|---|---|
| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), ANR-11-IDEX-0002,UNITI,Towards a Unified theory of biotic Interactions: the roLe of environmental(2011) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
RNA
Untranslated protein synthesis [SDV]Life Sciences [q-bio] Arabidopsis Ribosome biogenesis plant Biology 03 medical and health sciences 0302 clinical medicine RNA Transfer Gene Expression Regulation Plant Molecular Biology 030304 developmental biology Regulation of gene expression 0303 health sciences Cell Biology Rna degradation biology.organism_classification tRNAs Cell biology polyribosomes 030220 oncology & carcinogenesis Protein Biosynthesis small non-coding RNAs Transfer RNA Nucleic Acid Conformation Research Paper |
| Zdroj: | RNA Biology RNA Biology, Taylor & Francis, 2020, ⟨10.1080/15476286.2020.1722514⟩ RNA Biol |
| ISSN: | 1547-6286 1555-8584 |
| Popis: | Transfer RNA-derived fragments (tRFs) exist in all branches of life. They are involved in RNA degradation, regulation of gene expression, ribosome biogenesis. In archaebacteria, kinetoplastid, yeast, and human cells, they were also shown to regulate translation. In Arabidopsis, the tRFs population fluctuates under developmental or environmental conditions but their functions are yet poorly understood. Here, we show that populations of long (30–35 nt) or short (19–25 nt) tRFs produced from Arabidopsis tRNAs can inhibit in vitro translation of a reporter gene. Analysing a series of oligoribonucleotides mimicking natural tRFs, we demonstrate that only a limited set of tRFs possess the ability to affect protein synthesis. Out of a dozen of tRFs, only two deriving from tRNAAla(AGC) and tRNAAsn(GUU) strongly attenuate translation in vitro. Contrary to human tRF(Ala), the 4 Gs present at the 5ʹ extremity of Arabidopsis tRF(Ala) are not implicated in this inhibition while the G18 and G19 residues are essential. Protein synthesis inhibition by tRFs does not require complementarity with the translated mRNA but, having the capability to be associated with polyribosomes, tRFs likely act as general modulation factors of the translation process in plants. |
| Databáze: | OpenAIRE |
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