Production of early and late nuclear DNA damage and extracellular 8- oxodG in normal human skin fibroblasts after carbon ion irradiation compared to X-rays
| Autor: | Stéphanie Lagadu, François Sichel, Virginie Prevost, Alexandre Leduc, Ivannah Pottier, Carine Laurent |
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| Přispěvatelé: | Unité de recherche interdisciplinaire pour la prévention et le traitement des cancers (ANTICIPE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)-Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU), Aliments Bioprocédés Toxicologie Environnements (ABTE), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Université de Rouen Normandie (UNIROUEN), SAPHYN (SAnté et PHYsique Nucléaire), This work was supported by grants from Electricité de France and the Ligue contre le Cancer., Prevost, Virginie |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
skin DNA damage DNA repair Binucleated cells [SDV]Life Sciences [q-bio] 8-oxodG Human skin Toxicology medicine.disease_cause Young Adult 03 medical and health sciences 0302 clinical medicine medicine Humans oxidative stress Heavy Ions Cells Cultured Micronuclei Chromosome-Defective ComputingMilieux_MISCELLANEOUS Micronucleus Tests Chemistry X-Rays Deoxyguanosine General Medicine Fibroblasts Molecular biology Carbon [SDV] Life Sciences [q-bio] carbon ions 030104 developmental biology 8-Hydroxy-2'-Deoxyguanosine 030220 oncology & carcinogenesis Micronucleus test Micronucleus Oxidative stress Genotoxicity |
| Zdroj: | Toxicology in Vitro Toxicology in Vitro, Elsevier, 2018, 52, pp.116-121. ⟨10.1016/j.tiv.2018.06.004⟩ |
| ISSN: | 0887-2333 |
| Popis: | This work aims to evaluate genotoxicity of C-ion vs. X-ray irradiation in normal human skin fibroblasts. Clonogenic cell survival was first evaluated using a linear-quadratic model. Then, early and late genotoxicity was quantified by alkaline comet assay, micronucleus test and 8-oxodG extracellular measurement. Survival strongly decreased after C-ions compared to X-rays with a 4.8-fold decrease at D 0 – irradiation dose corresponding to 37% of survival. The level of immediate DNA damage was approximately the same after C-ions or X-rays. However, half-time of DNA repair was 1.3-fold decreased after C-ions compared to X-rays leading to a 2.2-fold increase in remaining damage. In the same way, micronucleus formation was 1.7-fold increased 24 h after irradiation and remained 1.8-fold increased 2 weeks after C-ions vs. X-rays when secondary oxidative stress wave occurred. This was related to a 2.6-fold increase in binucleated cells percentage in carbon- vs. X-ray-irradiated fibroblasts. Excretion of 8-oxodG was also 1.5–fold increased after C-ions vs. X-rays. 8-oxodG excretion could therefore participate to the appearance of late waves of oxidative stress. These results showed a stronger genotoxicity of C-ions in skin fibroblasts. More investigations are needed to clarify the discrepancy between the two types of radiations. |
| Databáze: | OpenAIRE |
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