Dendritic LSm1/CBP80-mRNPs mark the early steps of transport commitment and translational control

Autor: di Penta A. 1, Mercaldo V. 1, 2, 3, Florenzano F. 1, Munck S. 2, Ciotti M.T. 4, Zalfa F. 1, 5, Mercanti D. 4, Molinari M. 1, Bagni C. 1, Achsel T. 1
Jazyk: angličtina
Rok vydání: 2009
Předmět:
fragile-x-syndrome
protein synthesis
protein synthesis regulation
Wistar
animal cell
Wistar rat
gene silencing
Mice
Cerebellum
Protein biosynthesis
LSm1 protein
genetics
spinal muscular-atrophy
Cells
Cultured

Neurons
Cultured
Settore BIO/13
cap binding protein
cap binding protein 80
glutamate receptor
messenger RNA
ribonucleoprotein
unclassified drug
biological marker
cleavage and polyadenylation specificity factor
CPEB1 protein
rat

LSm1 protein
rat

messenger ribonucleoprotein
nuclear cap binding protein
oncoprotein
ribonuclease
RNA binding protein
animal tissue
article
controlled study
dendrite
intracellular transport
male
mouse
nerve cell culture
nerve cell plasticity
nonhuman
priority journal
rat
RNA transport
translation regulation
animal
brain
cell culture
cerebellum
cytology
gene expression regulation
metabolism
mouse strain
nerve cell
physiology
spinal cord
transport at the cellular level
ultrastructure
Animals
Biological Markers
Biological Transport
Brain
Dendrites
Endoribonucleases
Gene Expression Regulation
Gene Silencing
Mice
Inbred Strains

mRNA Cleavage and Polyadenylation Factors
Nuclear Cap-Binding Protein Complex
Protein Biosynthesis
Proto-Oncogene Proteins
Rats
Rats
Wistar

Ribonucleoproteins
RNA
Messenger

RNA-Binding Proteins
Spinal Cord
dependent translation
Cell biology
Cells
CPEB1 protein
cytoplasmic polyadenylation
messenger-rna translation
Biomarkers
nonsense-mediated decay
Nonsense-mediated decay
Messenger
mammalian-cells
Inbred Strains
RNA-binding protein
Translational regulation
mrnp complex
Research Articles
medicine.anatomical_structure
processing bodies
Biology
local protein-synthesis
medicine
Messenger RNA
Nuclear cap-binding protein complex
Cell Biology
Molecular biology
Cell nucleus
Synaptic plasticity
RNA
Zdroj: The Journal of cell biology 184 (2009): 423–435. doi:10.1083/jcb.200807033
info:cnr-pdr/source/autori:di Penta A. 1, Mercaldo V. 1,2,3, Florenzano F. 1, Munck S. 2,3, Ciotti M.T. 4, Zalfa F. 1,5, Mercanti D. 4, Molinari M. 1, Bagni C. 1,2,3,5, Achsel T. 1,2,3/titolo:Dendritic LSm1%2FCBP80-mRNPs mark the early steps of transport commitment and translational control/doi:10.1083%2Fjcb.200807033/rivista:The Journal of cell biology/anno:2009/pagina_da:423/pagina_a:435/intervallo_pagine:423–435/volume:184
The Journal of Cell Biology
DOI: 10.1083/jcb.200807033
Popis: Messenger RNA (mRNA) transport to neuronal dendrites is crucial for synaptic plasticity, but little is known of assembly or translational regulation of dendritic messenger ribonucleoproteins (mRNPs). Here we characterize a novel mRNP complex that is found in neuronal dendrites throughout the central nervous system and in some axonal processes of the spinal cord. The complex is characterized by the LSm1 protein, which so far has been implicated in mRNA degradation in non-neuronal cells. In brain, it associates with intact mRNAs. Interestingly, the LSm1-mRNPs contain the cap-binding protein CBP80 that associates with (pre) mRNAs in the nucleus, suggesting that the dendritic LSm1 complex has been assembled in the nucleus. In support of this notion, neuronal LSm1 is partially nuclear and inhibition of mRNA synthesis increases its nuclear localization. Importantly, CBP80 is also present in the dendrites and both LSm1 and CBP80 shift significantly into the spines upon stimulation of glutamergic receptors, suggesting that these mRNPs are translationally activated and contribute to the regulated local protein synthesis. ispartof: Journal of Cell Biology vol:184 issue:3 pages:423-35 ispartof: location:United States status: published
Databáze: OpenAIRE