Dendritic LSm1/CBP80-mRNPs mark the early steps of transport commitment and translational control
Autor: | di Penta A. 1, Mercaldo V. 1, 2, 3, Florenzano F. 1, Munck S. 2, Ciotti M.T. 4, Zalfa F. 1, 5, Mercanti D. 4, Molinari M. 1, Bagni C. 1, Achsel T. 1 |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2009 |
Předmět: |
fragile-x-syndrome
protein synthesis protein synthesis regulation Wistar animal cell Wistar rat gene silencing Mice Cerebellum Protein biosynthesis LSm1 protein genetics spinal muscular-atrophy Cells Cultured Neurons Cultured Settore BIO/13 cap binding protein cap binding protein 80 glutamate receptor messenger RNA ribonucleoprotein unclassified drug biological marker cleavage and polyadenylation specificity factor CPEB1 protein rat LSm1 protein rat messenger ribonucleoprotein nuclear cap binding protein oncoprotein ribonuclease RNA binding protein animal tissue article controlled study dendrite intracellular transport male mouse nerve cell culture nerve cell plasticity nonhuman priority journal rat RNA transport translation regulation animal brain cell culture cerebellum cytology gene expression regulation metabolism mouse strain nerve cell physiology spinal cord transport at the cellular level ultrastructure Animals Biological Markers Biological Transport Brain Dendrites Endoribonucleases Gene Expression Regulation Gene Silencing Mice Inbred Strains mRNA Cleavage and Polyadenylation Factors Nuclear Cap-Binding Protein Complex Protein Biosynthesis Proto-Oncogene Proteins Rats Rats Wistar Ribonucleoproteins RNA Messenger RNA-Binding Proteins Spinal Cord dependent translation Cell biology Cells CPEB1 protein cytoplasmic polyadenylation messenger-rna translation Biomarkers nonsense-mediated decay Nonsense-mediated decay Messenger mammalian-cells Inbred Strains RNA-binding protein Translational regulation mrnp complex Research Articles medicine.anatomical_structure processing bodies Biology local protein-synthesis medicine Messenger RNA Nuclear cap-binding protein complex Cell Biology Molecular biology Cell nucleus Synaptic plasticity RNA |
Zdroj: | The Journal of cell biology 184 (2009): 423–435. doi:10.1083/jcb.200807033 info:cnr-pdr/source/autori:di Penta A. 1, Mercaldo V. 1,2,3, Florenzano F. 1, Munck S. 2,3, Ciotti M.T. 4, Zalfa F. 1,5, Mercanti D. 4, Molinari M. 1, Bagni C. 1,2,3,5, Achsel T. 1,2,3/titolo:Dendritic LSm1%2FCBP80-mRNPs mark the early steps of transport commitment and translational control/doi:10.1083%2Fjcb.200807033/rivista:The Journal of cell biology/anno:2009/pagina_da:423/pagina_a:435/intervallo_pagine:423–435/volume:184 The Journal of Cell Biology |
DOI: | 10.1083/jcb.200807033 |
Popis: | Messenger RNA (mRNA) transport to neuronal dendrites is crucial for synaptic plasticity, but little is known of assembly or translational regulation of dendritic messenger ribonucleoproteins (mRNPs). Here we characterize a novel mRNP complex that is found in neuronal dendrites throughout the central nervous system and in some axonal processes of the spinal cord. The complex is characterized by the LSm1 protein, which so far has been implicated in mRNA degradation in non-neuronal cells. In brain, it associates with intact mRNAs. Interestingly, the LSm1-mRNPs contain the cap-binding protein CBP80 that associates with (pre) mRNAs in the nucleus, suggesting that the dendritic LSm1 complex has been assembled in the nucleus. In support of this notion, neuronal LSm1 is partially nuclear and inhibition of mRNA synthesis increases its nuclear localization. Importantly, CBP80 is also present in the dendrites and both LSm1 and CBP80 shift significantly into the spines upon stimulation of glutamergic receptors, suggesting that these mRNPs are translationally activated and contribute to the regulated local protein synthesis. ispartof: Journal of Cell Biology vol:184 issue:3 pages:423-35 ispartof: location:United States status: published |
Databáze: | OpenAIRE |
Externí odkaz: |