Concanavalin-A Induces Granulosa Cell Death and Inhibits FSH-Mediated Follicular Growth and Ovarian Maturation in Female Rats
| Autor: | Ricardo D. Moreno, Mariana Ríos, Anne Rokka, Dalhia Abramovich, Manuel Villalón, Marta Tesone, Barbara Oliva, Elizabeth Nuñez, Felipe Orge, Garry L. Corthals, M. E. Ortiz, Horacio B. Croxatto, Fernanda Parborell, Renan Orellana, Gareth I. Owen, Carlos Lizama, Ethel V. Velasquez |
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| Rok vydání: | 2013 |
| Předmět: |
endocrine system
medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Granulosa cell education Ciencias de la Salud Apoptosis Biology Rats Sprague-Dawley purl.org/becyt/ford/3.3 [https] Endocrinology Ovarian Follicle Internal medicine parasitic diseases Fsh Follicular phase Concanavalin A medicine Animals Sexual Maturation Ovarian follicle Cells Cultured Granulosa Cells Cell Death Ovary Rats Otras Ciencias de la Salud medicine.anatomical_structure biology.protein population characteristics Female purl.org/becyt/ford/3 [https] Follicle Stimulating Hormone Mitogens geographic locations |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET ENDOCRINOLOGY Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | Reproductive success stems from a finely regulated balance between follicular maturation and atresia, in which the role of carbohydrate structure is poorly understood. Here, we describe for the first time a fraction of purified recombinant human FSH that is capable of bringing about the cell death of granulosa cells and preventing follicular maturation in a rat model. Further analysis by mass spectrometry revealed the presence of the lectin Concanavalin-A (Con-A) within this fraction of recombinant FSH. Using both the fractionated FSH and Con-A, the observed cell death was predominantly located to the granulosa cells. Ex vivo culture of rat follicles demonstrated that follicle degeneration occurred and resulted in the release of a denuded and deteriorated oocyte. Moreover, in vivo experiments confirmed an increase in atresia and a corresponding reduction confined to follicle in early antral stage. As a mechanism of action, Con-A reduces ovarian proliferation, Von Willebrand staining, and angiogenesis. Based on the observation that Con-A may induce granulosa cell death followed by follicle death, our results further demonstrate that follicular carbohydrate moiety is changing under the influence of FSH, which may allow a carbohydrate-binding lectin to increase granulosa cell death. The physiological consequences of circulating lectin-like molecules remain to be determined. However, our results suggest a potential exploitation of carbohydrate binding in fertility and ovarian cancer treatment. This work may shed light on a key role of carbohydrates in the still obscure physiological process of follicular selection and atresia. Fil: Velasquez, Ethel V.. Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas; Chile; Universidad de Santiago de Chile. Facultad de Química y Biología; Chile; instituto Chileno de Medicina Reproductiva; Chile; Fil: Rios, Mariana. Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas; Chile; Fil: Ortiz, María Elena. Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas; Chile; Instituto Chileno de Medicina Reproductiva; Chile; Fil: Lizama, Carlos. Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas; Chile; Fil: Nuñez, Elizabeth. Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas; Chile; Fil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Fil: Orge, Felipe. Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas; Chile; Fil: Oliva, Bárbara. Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas; Chile; Fil: Orellana, Renán. Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas; Chile; Fil: Villalon, Manuel. Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas; Chile; Fil: Moreno, Ricardo D.. Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas; Chile; Fil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Fil: Rokka, Anne. Turku Centre for Biotechnology; Finlandia; Universidad Nacional Andrés Bello. Facultad de Medicina; Chile; Fil: Corthals, Garry. Turku Centre for Biotechnology; Finlandia; Universidad Nacional Andrés Bello. Facultad de Medicina; Chile; Fil: Croxatto, Horacio B.. Universidad de Santiago de Chile. Facultad de Química y Biología; Chile; Fil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Fil: Owen, Gareth I.. Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas; Chile; Instituto Chileno de Medicina Reproductiva; Chile |
| Databáze: | OpenAIRE |
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