Insights from capillary electrophoresis approaches for characterization of monoclonal antibodies and antibody drug conjugates in the period 2016–2018

Autor: Alain Beck, Jérémie Giorgetti, Rabah Gahoual, Antony Lechner, Emmanuelle Leize-Wagner, Yannis-Nicolas François
Přispěvatelé: Tectonique Moléculaire du Solide (TMS), Chimie de la matière complexe (CMC), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre d'Immunologie Pierre Fabre, Laboratoire de synthèses métallo-induites, Dynamique et structure moléculaire par spectrométrie de masse (LDSM2), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2019
Předmět:
Drug
Antibody-drug conjugate
Immunoconjugates
medicine.drug_class
media_common.quotation_subject
Clinical Biochemistry
Context (language use)
Capillary Gel Electrophoresis
Monoclonal antibody
030226 pharmacology & pharmacy
01 natural sciences
Biochemistry
Analytical Chemistry
03 medical and health sciences
0302 clinical medicine
Capillary electrophoresis
Antibody Drug Conjugate
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
medicine
Humans
Capillary Zone Electrophoresis
media_common
Chromatography
Mass spectrometry
Isoelectric focusing
Chemistry
Biosimilar
010401 analytical chemistry
Antibodies
Monoclonal
Electrophoresis
Capillary
Cell Biology
General Medicine
Capillary Isoelectric Focusing
0104 chemical sciences
Characterization (materials science)
Monoclonal antibodies
Isoelectric Focusing
Micro-fluidic capillary electrophoresis
Post-translational modifications
Zdroj: Journal of Chromatography B Biomedical Sciences and Applications
Journal of Chromatography B Biomedical Sciences and Applications, Elsevier, 2019, 1122-1123, pp.1-17. ⟨10.1016/j.jchromb.2019.05.014⟩
ISSN: 1570-0232
0378-4347
DOI: 10.1016/j.jchromb.2019.05.014
Popis: International audience; Monoclonal antibodies (mAbs) and their related products as antibody-drug-conjugates (ADCs) or biosimilars represent a constantly growing class of molecules therapeutic proteins used as treatment against numerous diseases. These compounds can undergo several modifications which could alter the efficiency of treatments. In this context, several analytical methods were designed to deliver a comprehensive structural characterization and guarantee the quality of biotherapeutics. Capillary electrophoresis (CE) is considered today as a major technique for the analysis of biotherapeutics due to benefic characteristics as high resolution separation and miniaturized format. Different CE modes have been developed to characterize mAbs at different levels such as capillary gel electrophoresis (CGE), capillary isoelectric focusing (cIEF), and capillary zone electrophoresis (CZE). Recent developments in CE-mass spectrometry (MS) coupling assessed this technology as a promising tool to obtain high level structural characterization of biopharmaceuticals. Moreover, upcoming techniques such as 2D CE-MS and microfluidic systems are now emerging to offer new possibilities beyond actual limits. This review will be dedicated to discuss the state-of-the-art CE-based methods for the characterization of mAbs and ADCs in the period 2016-2018.
Databáze: OpenAIRE
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