miR-33a-5p modulates TNF-α-inhibited osteogenic differentiation by targeting SATB2 expression in hBMSCs

Autor: Wenxiang Mi, Qiongling Shi, Tingting Wu, Xipeng Chen, Hui Huang
Rok vydání: 2015
Předmět:
0301 basic medicine
Male
Biophysics
Bone Morphogenetic Protein 2
Inflammation
Apoptosis
Bone Marrow Cells
Core Binding Factor Alpha 1 Subunit
Biology
Biochemistry
Bone morphogenetic protein 2
03 medical and health sciences
Mice
0302 clinical medicine
Structural Biology
Genes
Reporter
Osteogenesis
microRNA
Genetics
medicine
Animals
Humans
Molecular Biology
Transcription factor
Cells
Cultured
Cell Proliferation
Cell growth
Tumor Necrosis Factor-alpha
Gene Expression Regulation
Developmental
Cell Biology
Matrix Attachment Region Binding Proteins
Molecular biology
Recombinant Proteins
Cell biology
Blot
MicroRNAs
030104 developmental biology
030220 oncology & carcinogenesis
Tumor necrosis factor alpha
Female
medicine.symptom
Signal transduction
Stromal Cells
Signal Transduction
Transcription Factors
Zdroj: FEBS letters. 590(3)
ISSN: 1873-3468
Popis: miRNAs play a number of roles in bone, including mediating the pathological effects of inflammation. Here, we found that miR-33a-5p expression was significantly increased after TNF-α treatment during BMP-2-induced osteogenic differentiation of hBMSCs. Luciferase reporter assays and western blotting demonstrated that special AT-rich sequence-binding protein 2 (SATB2) is a target of miR-33a-5p. Moreover, we show that BMP-2 induces SATB2 expression by interacting with SATB2 directly via the BMP-2-RUNX2 pathway. However, TNF-α first decreases SATB2 expression by inhibiting miR-33a-5p degradation. We thus conclude that miR-33a-5p plays a central role in this complex regulatory network. These findings will help to understand the regulatory role of miR-33a-5p in the inflammatory process.
Databáze: OpenAIRE
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