DNA methylation detection at single base resolution using targeted next generation bisulfite sequencing and cross validation using capillary sequencing
| Autor: | Sandeep Mallya, Kalpana Joshi, Vinay Koshy Varghese, Tanuja M. Nesari, Sanjiban Chakrabarty, Pradyumna Jayaram, Kapaettu Satyamoorthy, Smitha Bhat |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Bisulfite sequencing High-Throughput Nucleotide Sequencing Sequence Analysis DNA General Medicine Methylation DNA Methylation Biology Molecular biology 03 medical and health sciences genomic DNA 030104 developmental biology Differentially methylated regions CpG site Genetic Loci DNA methylation Genetics Humans Illumina Methylation Assay CpG Islands Female Methylated DNA immunoprecipitation |
| Zdroj: | Gene. 594:259-267 |
| ISSN: | 0378-1119 |
| Popis: | With a purpose of accurate and simultaneous determination of DNA methylation from multiple loci in multiple samples, here, we are demonstrating a method to aid rapid DNA methylation detection of genomic sequences. Using genomic DNA of peripheral blood from 14 healthy individuals, DNA methylation in 465 CpG sites from 12 loci of genes (ADAM22, ATF2, BCR, CD83, CREBBP, IL12B, IL17RA, MAP2K2, RBM38, TGFBR2, TGFBR3, and WNT5A) was analysed by targeted next generation bisulfite sequencing. Analysed region for three genes, BCR, IL17RA and RBM38 showed an absolute mean DNA methylation of 25.6%, 89.2% and 38.9% respectively. Other nine gene loci were unmethylated and exhibited |
| Databáze: | OpenAIRE |
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