Amplified-fragment length polymorphism analysis of Propionibacterium isolates implicated in contamination of blood products
| Autor: | R. N. I. Pietersz, Paul H. M. Savelkoul, H. W. Reesink, T. Mohammadi, C. M. J. E. Vandenbroucke-Grauls |
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| Přispěvatelé: | VU University medical center, Amsterdam Gastroenterology Endocrinology Metabolism, Gastroenterology and Hepatology, Amsterdam institute for Infection and Immunity, Medical Microbiology and Infection Prevention |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Blood Platelets
DNA Bacterial Amplified Fragment Length Polymorphism Analysis biology Genotype Propionibacterium Skin flora Reproducibility of Results Hematology biology.organism_classification 16S ribosomal RNA Molecular biology DNA Fingerprinting Bacterial Typing Techniques Propionibacterium acnes Blood DNA profiling Humans Amplified fragment length polymorphism Blood Transfusion Nucleic Acid Amplification Techniques |
| Zdroj: | Mohammadi, T, Reesink, H W, Pietersz, R N, Vandenbroucke-Grauls, C M J E & Savelkoul, P H M 2005, ' Amplified-fragment length polymorphism analysis of Propionibacterium isolates implicated in contamination of blood products ', British Journal of Haematology, vol. 131, no. 3, pp. 403-409 . https://doi.org/10.1111/j.1365-2141.2005.05771.x British Journal of Haematology, 131(3), 403-409. Wiley-Blackwell British journal of haematology, 131(3), 403-409. Wiley-Blackwell |
| ISSN: | 0007-1048 |
| DOI: | 10.1111/j.1365-2141.2005.05771.x |
| Popis: | Summary Propionibacterium acnes is implicated in most cases of bacterial contamination of platelet concentrates (PCs). To determine the source of contamination, amplified-fragment length polymorphism (AFLP) analysis was applied. This DNA fingerprinting technique was used to study the molecular relationship of 44 isolates derived from 22 PCs and 22 corresponding red blood cells concentrates (RBCs) from the same whole blood donations. The AFLP results together with sequencing analysis of the 1200 bp of the 16S ribosomal RNA gene revealed the existence of three main groups: two groups (groups 2 and 3) (55%) consisted of isolates that did not originate from skin flora and another group (group 1) (45%) comprised bacteria belonging to the skin flora. This latter group showed complete homology with reference strains of P. acnes. Therefore these isolates can be considered as P. acnes strains. In contrast, contaminants from groups 2 and 3 were shown to be molecularly unrelated to the P. acnes found on the skin surface. The AFLP is reproducible and gave invaluable information about the nature of Propionibacteria contaminating PCs. To gain more insights into the source of contamination, this technique could be exploited in further studies to determine the molecular relationship of different bacteria commonly found in blood products. |
| Databáze: | OpenAIRE |
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