A hemoprotein from azotobacter containing non-heme iron: Isolation and crystallization
| Autor: | William A. Bulen, J.R. LeComte, Samuel M. Lough |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Hemeproteins
inorganic chemicals Hemeprotein Cytochrome Iron Inorganic chemistry Biophysics chemistry.chemical_element Heme Biochemistry Redox law.invention chemistry.chemical_compound law Polymer chemistry Crystallization Molecular Biology Azotobacter biology Cell Biology biology.organism_classification Sulfur chemistry Azotobacter vinelandii Spectrophotometry biology.protein Cytochromes Electrophoresis Polyacrylamide Gel Oxidation-Reduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 54:1274-1281 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(73)91125-x |
| Popis: | A new type of hemoprotein was isolated from extracts of Azotobacter vinelandii using a heat step, application of the protamine-cellulose phosphate procedure, and precipitation with MgCl2. Crystallization was achieved using low concentration of MgCl2. The protein was shown to be a unique cytochrome of the b type containing up to 20% non-heme iron but no “acid labile sulfur”. Both the heme and non-heme iron appear to be functional redox components. |
| Databáze: | OpenAIRE |
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