Intermembrane transfer of polyethylene glycol-modified phosphatidylethanolamine as a means to reveal surface-associated binding ligands on liposomes
| Autor: | Lawrence D. Mayer, Lanny Xue, Marcel B. Bally, Wai Ming Li |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Streptavidin
Time Factors Surface Properties Biophysics Biotin Polyethylene glycol Glycerophospholipids Ligands Micelle Biochemistry Ferric Compounds Polyethylene Glycols 03 medical and health sciences chemistry.chemical_compound Mice Intermembrane transfer PEG ratio Animals Ligand binding Micelles 030304 developmental biology 0303 health sciences Liposome Chromatography Poly(ethylene glycol) Phosphatidylethanolamines 030302 biochemistry & molecular biology technology industry and agriculture Cell Biology Ligand (biochemistry) chemistry Biotinylation Injections Intravenous Liposomes lipids (amino acids peptides and proteins) Ethylene glycol Nuclear chemistry |
| Zdroj: | Biochimica et biophysica acta. 1513(2) |
| ISSN: | 0006-3002 |
| Popis: | In order to explore the use of exchangeable poly(ethylene glycol) (PEG)-modified diacylphosphatidylethanolamines (PE) to temporarily shield binding ligands attached to the surface of liposomes, a model reaction based on inhibition and subsequent recovery of biotinylated liposome binding to streptavidin immobilized on superparamagnetic iron oxide particles (SA magnetic particles) was developed. PEG-lipid incorporation into biotinylated liposomes decreased liposome binding to SA magnetic particles in a non-linear fashion, where as little as 0.1 mol% PEG-PE resulted in a 20% decrease in binding. Using an assay based on inhibition of binding, PEG(2000)-PE transfer from donor liposomes to biotinylated acceptor liposomes could be measured. The influence of temperature and acyl chain composition on the transfer of PEG-diacyl PEs from donor liposomes to acceptor liposomes, consisting of 1,2-dioleoyl-sn-glycero-3-phosphocholine, cholesterol and N-((6-biotinoyl)amino)hexanoyl)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (54.9:45:0.1 mole ratio), was measured. Donor liposomes were prepared using 1,2-distearoyl-sn-glycero-3-phosphocholine (50 mol%), cholesterol (45 mol%) and 5 mol% of either PEG-derivatized 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE-PEG(2000)), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE-PEG(2000)), or 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE-PEG(2000)). Transfer of DSPE-PEG(2000) to the donor liposomes was not detected under the conditions employed. In contrast, DMPE-PEG(2000) was transferred efficiently even at 4 degrees C. Using an acceptor to donor liposome ratio of 1:4, the time required for DMPE-PEG(2000) to become evenly distributed between the two liposome populations (T(EQ)) at 4 degrees C and 37 degrees C was approx. 2 and |
| Databáze: | OpenAIRE |
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