Investigation of synovial fluid lubricants and inflammatory cytokines in the horse: a comparison of recombinant equine interleukin 1 beta-induced synovitis and joint lavage models
| Autor: | Katherine Caracappa, Bettina Wagner, Amanda R. Watkins, Albert D’Agostino, Marshall J. Colville, Heather Freer, Sydney Schurer, Matthew J. Paszek, Diana C. Fasanello, Claire Read, Heidi L. Reesink, Jin Su, Darko Stefanovski, Emily Costello, Alicia Rollins |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Pathology medicine.medical_treatment Veterinary medicine Interleukin-1beta Osteoarthritis Injections Intra-Articular 0403 veterinary science chemistry.chemical_compound Hyaluronic acid Synovial Fluid Lubrication SF600-1100 0303 health sciences Synovitis biology 04 agricultural and veterinary sciences General Medicine medicine.anatomical_structure Chemokine Cytokines Female medicine.symptom Rheology Arthrocentesis medicine.medical_specialty 040301 veterinary sciences 03 medical and health sciences Proteoglycan 4 medicine Synovial fluid Animals Horses Therapeutic Irrigation 030304 developmental biology Glycoproteins Inflammation General Veterinary business.industry Research Joint effusion medicine.disease chemistry biology.protein Lubricin Horse Diseases Synovial membrane business Repeated arthrocentesis |
| Zdroj: | BMC Veterinary Research, Vol 17, Iss 1, Pp 1-18 (2021) BMC Veterinary Research |
| ISSN: | 1746-6148 |
| Popis: | Background Lameness is a debilitating condition in equine athletes that leads to more performance limitation and loss of use than any other medical condition. There are a limited number of non-terminal experimental models that can be used to study early inflammatory and synovial fluid biophysical changes that occur in the equine joint. Here, we compare the well-established carpal IL-1β-induced synovitis model to a tarsal intra-articular lavage model, focusing on serial changes in synovial fluid inflammatory cytokines/chemokines and the synovial fluid lubricating molecules lubricin/proteoglycan 4 and hyaluronic acid. The objectives of this study were to evaluate clinical signs; synovial membrane and synovial fluid inflammation; and synovial fluid lubricants and biophysical properties in response to carpal IL-1β synovitis and tarsal intra-articular lavage. Results Hyaluronic acid (HA) concentrations, especially high molecular weight HA, and synovial fluid viscosity decreased after both synovitis and lavage interventions. Synovial fluid lubricin concentrations increased 17–20-fold for both synovitis and lavage models, with similar changes in both affected and contralateral joints, suggesting that repeated arthrocentesis alone resulted in elevated synovial fluid lubricin concentrations. Synovitis resulted in a more severe inflammatory response based on clinical signs (temperature, heart rate, respiratory rate, lameness and joint effusion) and clinicopathological and biochemical parameters (white blood cell count, total protein, prostaglandin E2, sulfated glycosaminoglycans, tumor necrosis factor-α and CC chemokine ligands − 2, − 3, − 5 and − 11) as compared to lavage. Conclusions Synovial fluid lubricin increased in response to IL-1β synovitis and joint lavage but also as a result of repeated arthrocentesis. Frequent repeated arthrocentesis is associated with inflammatory changes, including increased sulfated glycosaminoglycan concentrations and decreased hyaluronic acid concentrations. Synovitis results in more significant inflammatory changes than joint lavage. Our data suggests that synovial fluid lubricin, TNF-α, CCL2, CCL3, CCL5, CCL11 and sGAG may be useful biomarkers for synovitis and post-lavage joint inflammation. Caution should be exercised when performing repeated arthrocentesis clinically or in experimental studies due to the inflammatory response and loss of HA and synovial fluid viscosity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|