Determination of atracurium and laudanosine in human plasma by high-performance liquid chromatography
| Autor: | Besner Jg, Yves Théorêt, James Ducharme, F. Varin |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Chromatography
Fluorescence spectrometry General Chemistry Isoquinolines High-performance liquid chromatography Blood proteins Laudanosine chemistry.chemical_compound Spectrometry Fluorescence chemistry Pharmacokinetics Human plasma Atracurium Humans Indicators and Reagents Quantitative analysis (chemistry) Chromatography High Pressure Liquid Half-Life |
| Zdroj: | Journal of chromatography. 529(2) |
| Popis: | A high-performance liquid chromatographic method coupled with fluorometric detection has been developed for the determination of atracurium and its major end-product laudanosine in human plasma. The method enables good separation of atracurium from its metabolites after direct precipitation of plasma proteins. The assay is sensitive, reproducible and linear for atracurium concentrations ranging from 31.25 to 8000 ng/ml. In a clinical setting, drugs commonly administered during anesthesia did not interfere with the assay. This method provides a simple and time-saving alternative to existing methods. |
| Databáze: | OpenAIRE |
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