A non-canonical phosphorylation site in β-casein A from non-Mediterranean water buffalo makes quantifiable the adulteration of Italian milk with foreign material by combined isoelectrofocusing-immunoblotting procedures
| Autor: | Gabriella Pinto, Maria Adalgisa Nicolai, Andrea Scaloni, Simonetta Caira, Gianfranco Novi, Francesco Addeo |
|---|---|
| Přispěvatelé: | Caira, Simonetta, Pinto, Gabriella, Nicolai, MARIA ADALGISA, Novi, Gianfranco, Addeo, Francesco, Scaloni, Andrea |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Phosphorylation sites Buffaloes Immunoblotting Proteomics Milk Isoelectrofocusing Immunoblotting Protein markers 01 natural sciences Analytical Chemistry 0404 agricultural biotechnology Protein markers Casein Isoelectrofocusing Food Quality Animals Food science Phosphorylation Detection limit Binding Sites Chemistry 010401 analytical chemistry Fraud Caseins 04 agricultural and veterinary sciences General Medicine 040401 food science 0104 chemical sciences Linear relationship Milk β casein Non canonical Water buffalo Isoelectric Focusing Biomarkers Food Science |
| Zdroj: | Food chemistry 277 (2019): 195–204. doi:10.1016/j.foodchem.2018.10.076 info:cnr-pdr/source/autori:Caira S.; Pinto G.; Nicolai M.A.; Novi G.; Addeo F.; Scaloni A./titolo:A non-canonical phosphorylation site in beta-casein A from non-Mediterranean water buffalo makes quantifiable the adulteration of Italian milk with foreign material by combined isoelectrofocusing-immunoblotting procedures/doi:10.1016%2Fj.foodchem.2018.10.076/rivista:Food chemistry/anno:2019/pagina_da:195/pagina_a:204/intervallo_pagine:195–204/volume:277 |
| DOI: | 10.1016/j.foodchem.2018.10.076 |
| Popis: | The need of controlling illegal addition of water buffalo (WB) milk from foreign countries to the Italian counterpart devoted to the production of Protected Denomination of Origin (PDO) Mozzarella di Bufala Campana (MBC) cheese has promoted the development of simple, fast and cheap isoelectrofocusing (IEF) methods for evaluating the nature of the raw material to be used according to a high-throughput sample multiplexing format, avoiding the use of dedicated mass spectrometry-based procedures. Thus, combined proteomic methods were here integrated with optimized western blotting protocols in solving the complex IEF pattern of casein (CN) mixtures observed when Italian and foreign WB milk are mixed together. Identification of internally deleted αs1-CN hepta-phosphorylated species as well as of still unknown β-CN A hexa-phosphorylated and N-terminally-nicked β-CN A phosphorylated forms present uniquely in foreign WB milk samples, allowed recognizing these molecules as adulteration markers to be assayed in combined IEF-immunoblotting procedures; the latter ones showing optimal migration characteristics to be used in routine assays. A linear relationship between detected area of specific immunorecognized gel bands and percentage of international WB milk added to the Italian counterpart was verified, demonstrating that this method has an adulteration detection limit close to 3% v/v. Based on these results, this analytical procedure is here proposed as optimal one for evaluating the authenticity of PDO MBC cheese products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|