The circulating level of FABP3 is an indirect biomarker of microRNA-1
| Autor: | Gloria Saccani Jotti, Carolina Di Somma, Anna Franzone, Pierluigi Mauri, Barbara Gargano, Annamaria Colao, Antonella De Palma, Louise Benazzi, Gianluigi Condorelli, Pierluigi Carullo, Giovanni Esposito, Marie Louise Bang, Dario Di Silvestre, Ludovica F S Grasso, Francesca Varrone, Daniele Catalucci |
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| Přispěvatelé: | Varrone, F, Gargano, B, Carullo, P, Di Silvestre, D, De Palma, A, Grasso, Lf, DI SOMMA, Carolina, Mauri, P, Benazzi, L, Franzone, A, Jotti, G, Bang, Ml, Esposito, Giovanni, Colao, A, Condorelli, G, Catalucci, D. |
| Rok vydání: | 2012 |
| Předmět: |
Genetically modified mouse
medicine.medical_specialty FABP3 Enzyme-Linked Immunosorbent Assay Fatty Acid-Binding Proteins Growth hormone deficiency Mice Ventricular hypertrophy In vivo Internal medicine microRNA medicine Animals Humans Myocytes Cardiac RNA Messenger Insulin-Like Growth Factor I Cells Cultured biomarker cardiovascular diseases miR-1 Acromegaly Aortic Valve Stenosis Biomarkers Disease Models Animal Fasting Fatty Acid Binding Protein 3 Human Growth Hormone Hypertrophy Left Ventricular MicroRNAs Myocardium Reverse Transcriptase Polymerase Chain Reaction Cardiology and Cardiovascular Medicine business.industry medicine.disease Endocrinology Heart failure Ventricular pressure Biomarker (medicine) business |
| Zdroj: | Journal of the American College of Cardiology. 61(1) |
| ISSN: | 1558-3597 |
| Popis: | Objectives This study sought to identify proteins from the cardiomyocyte (CM) secretome that are directly targeted by the muscle-specific microRNA-1 (miR-1), and thus reflect the pathophysiological state of the CM. Background MicroRNAs play critical regulatory roles during myocardial remodeling and progression to heart failure. However, it remains unknown whether secreted microRNA-targeted proteins can be used as indicators of myocardial microRNA expression and function. Methods A proteomic analysis based on multidimensional protein identification technology was performed on supernatants from cultured CMs overexpressing miR-1. Biochemical assays and an inducible cardiac-specific transgenic mouse model overexpressing miR-1 were used to demonstrate that heart-type fatty acid-binding protein-3 (FABP3) is a target of miR-1. Levels of miR-1 and FABP3 in cardiac tissue and plasma samples from mouse models as well as human patients were quantified by quantitative reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The study included wild-type mice subjected to ventricular pressure overload or fasting, as well as patients diagnosed with ventricular hypertrophy due to valvular aortic stenosis, acromegaly, or growth hormone deficiency, conditions associated with altered miR-1 expression. Results An inverse relationship between myocardial expression of miR-1 and circulating levels of FABP3 was found both in vitro and in vivo under various pathological conditions. Conclusions Assessment of FABP3 plasma levels in human patients might be used for indirectly measuring cardiac miR-1 activity. |
| Databáze: | OpenAIRE |
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