Evaluation of the Andromas Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Aerobically Growing Gram-Positive Bacilli
| Autor: | Olivier Join-Lambert, M. Barritault, Anne Leflèche, Xavier Nassif, Carlos Vay, Emmanuelle Bergeron, S. Vimont, Jean-Luc Beretti, A.-S. Le Guern, Brunhilde Dauphin, Josette Raymond, Hélène Guet-Revillet, Emmanuelle Bille, Marc Lecuit, A. Le Monnier, Claudia Barberis, Alexandre Leclercq, E. Farfour, Veronica Rodriguez-Nava, E. Carbonnelle, Edgar Badell, Julie Meyer, Hervé Lécuyer, Nicole Guiso, Patrick Berche, J. Leto, Agnès Ferroni |
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| Přispěvatelé: | Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Bacteriological Techniques 0303 health sciences Bacilli Time Factors biology 030306 microbiology [SDV]Life Sciences [q-bio] Corynebacterium Bacteriology Nocardia Erysipelothrix rhusiopathiae Gram-Positive Bacteria biology.organism_classification Mass spectrometry Sensitivity and Specificity Microbiology Bacteria Aerobic 03 medical and health sciences Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Listeria Humans Rhodococcus equi Actinomyces 030304 developmental biology |
| Zdroj: | Journal of Clinical Microbiology Journal of Clinical Microbiology, American Society for Microbiology, 2012, 50 (8), pp.2702-2707. ⟨10.1128/JCM.00368-12⟩ |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.00368-12 |
| Popis: | Matrix-associated laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) is a rapid and simple microbial identification method. Previous reports using the Biotyper system suggested that this technique requires a preliminary extraction step to identify Gram-positive rods (GPRs), a technical issue that may limit the routine use of this technique to identify pathogenic GPRs in the clinical setting. We tested the accuracy of the MALDI-TOF MS Andromas strategy to identify a set of 659 GPR isolates representing 16 bacterial genera and 72 species by the direct colony method. This bacterial collection included 40 C. diphtheriae , 13 C. pseudotuberculosis , 19 C. ulcerans , and 270 other Corynebacterium isolates, 32 L. monocytogenes and 24 other Listeria isolates, 46 Nocardia , 75 Actinomyces , 18 Actinobaculum , 11 Propionibacterium acnes , 18 Propionibacterium avidum , 30 Lactobacillus , 21 Bacillus , 2 Rhodococcus equi , 2 Erysipelothrix rhusiopathiae , and 38 other GPR isolates, all identified by reference techniques. Totals of 98.5% and 1.2% of non- Listeria GPR isolates were identified to the species or genus level, respectively. Except for L. grayi isolates that were identified to the species level, all other Listeria isolates were identified to the genus level because of highly similar spectra. These data demonstrate that rapid identification of pathogenic GPRs can be obtained without an extraction step by MALDI-TOF mass spectrometry. |
| Databáze: | OpenAIRE |
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