Antibacterial Activity of Mulinum spinosum Extracts against Slime-Producing Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus Isolated from Nasal Carriers
| Autor: | Tonn Carlos, Chiaramello Alejandra, Laciar Analía, Echenique Daniela, Rossomando Pedro, Alcaráz Lucía, Satorres Sara, Mattana Claudia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
food.ingredient Article Subject medicine.drug_class Antibiotics Colony Count Microbial lcsh:Medicine Microbial Sensitivity Tests Nose Biology medicine.disease_cause lcsh:Technology Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology Microbiology law.invention food law medicine Humans Agar Stratum spinosum lcsh:Science Staphylococcus aureus delta toxin Polymerase chain reaction General Environmental Science lcsh:T Plant Extracts lcsh:R Congo Red General Medicine biochemical phenomena metabolism and nutrition Methicillin-resistant Staphylococcus aureus Anti-Bacterial Agents carbohydrates (lipids) Genes Bacterial Staphylococcus aureus lcsh:Q Antibacterial activity Research Article Apiaceae |
| Zdroj: | The Scientific World Journal The Scientific World Journal, Vol 2014 (2014) |
| ISSN: | 2356-6140 |
| DOI: | 10.1155/2014/342143 |
| Popis: | Nasal carriers ofStaphylococcus aureusare important reservoirs with risk of developing endogenous infections or transmitting infections to susceptible individuals. Methicillin-resistantS. aureus(MRSA) are associated with higher rates of treatment failure. Some strains ofS. aureusproduce slime which is believed to make the microorganisms more resistant to antibiotics and host defenses. The antibacterial activity of ethyl acetate : n-hexane (EtOAc : HEX) extracts ofMulinum spinosum(5 : 95% EtOAc : HEX, 50 : 50% EtOAc : HEX, 70 : 30% EtOAc : HEX and mix 20 : 80/30 : 70% EtOAc : HEX, 50 : 50/70 : 30/100 : 0% EtOAc : HEX) were assayed against 3 slime-producingS. aureusstrains and 2 MRSA strains isolated from nasal carriers.S. aureusATCC 35556 slime-producing strain and MRSA ATCC 43300 strain were used as controls. The extracts were prepared using flash chromatography.M. spinosum5 : 95% AcOEt : HEX showed antibacterial effect against all slime-producing strains (MIC: 500 µg/mL) and the highest activity against MRSA strains (MIC: 500 to 1000 µg/mL). AllM. spinosumextracts assayed were active against slime-producingS. aureusand MRSA at doses between 500 and 4000 µg/mL. Both, slime-producingS. aureusand MRSA are highly contagious and hardly eradicated by antibiotic therapies. So, there is an increasing need to find new substances with the ability to inhibit these strains. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text