First phase of insulin secretion stimulated by glucose plus theophylline and inhibitory effect of somatostatin in genetically diabetic mice (C57BL/KsJ-mdb)
| Autor: | J. C. Basabe, M Arata, Pivetta Oh, L. M. Karabatas, L. Anaya, J. C. Cresto |
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| Rok vydání: | 1988 |
| Předmět: |
Male
Aging medicine.medical_specialty Ratón Endocrinology Diabetes and Metabolism medicine.medical_treatment In Vitro Techniques Biology Diabetes Mellitus Experimental Islets of Langerhans Mice Theophylline Internal medicine Diabetes mellitus Insulin Secretion Internal Medicine medicine Animals Insulin Insulin secretion Inhibitory effect Genetic Carrier Screening Homozygote medicine.disease Mice Mutant Strains In vitro Glucose Endocrinology Somatostatin Female medicine.drug |
| Zdroj: | Diabetologia. 31:375-378 |
| ISSN: | 1432-0428 0012-186X |
| DOI: | 10.1007/bf02341506 |
| Popis: | In a previous study in C57BL/KsJ mdb/mdb mice aged 4 to 12 days we observed a diminished first phase of glucose-induced insulin secretion in vitro, and alterations in the inhibitory effect of somatostatin on insulin secretion. This study explores, using perifused pancreatic slices, whether the reduced B-cell responsiveness to somatostatin in mdb/mdb mice can be overcome upon induction of a biphasic insulin release by using theophylline. Under these conditions our results show: (1) in mdb/mdb mice aged 4 to 6 days, the restoration of the first peak of insulin secretion overcomes the reduced B-cell sensitivity to somatostatin; and (2) in mdb/mdb mice aged 7 to 12 days, the addition of theophylline only causes a partial restoration of B-cell responsiveness to somatostatin, suggesting that other mechanisms could be involved in the progressive impairement of B-cell sensitivity to somatostatin inhibitory effect. |
| Databáze: | OpenAIRE |
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