Retinoid-related orphan receptor alpha controls the early steps of Purkinje cell dendritic differentiation
| Autor: | Jean Mariani, Jacques Mallet, Vanessa Gautheron, Isabelle Dusart, Sonja Janmaat, Fatiha Boukhtouche, Guilan Vodjdani |
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| Přispěvatelé: | Neurobiologie des processus adaptatifs (NPA), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Génétique moléculaire de la neurotransmission et des processus neurodégénératifs (LGMNPN), This research was supported by grants from Fondation pour la Recherche Médicale (F.B.), Fondation Lejeune (F.B.), and Le Groupement d'Intérêt Scientifique Institut de la Longévité (J.M.). |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Cellular differentiation
Genetic Vectors Purkinje cell Receptor Protein-Tyrosine Kinases Receptors Cytoplasmic and Nuclear Receptor tyrosine kinase-like orphan receptor Receptors Cell Surface Biology Receptor Tyrosine Kinase-like Orphan Receptors behavioral disciplines and activities dendritic regression Mice Mice Neurologic Mutants 03 medical and health sciences Organ Culture Techniques 0302 clinical medicine medicine Animals Receptor 030304 developmental biology Orphan receptor 0303 health sciences General Neuroscience Lentivirus lentiviral vector ROR [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences Cell Differentiation Nuclear Receptor Subfamily 1 Group F Member 1 Dendrites Articles differentiation Embryonic stem cell Kinetics medicine.anatomical_structure Nuclear receptor organotypic culture Purkinje cells Trans-Activators Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neuroscience Journal of Neuroscience, Society for Neuroscience, 2006, 26, pp.1531-1538. ⟨10.1523/JNEUROSCI.4636-05.2006⟩ Journal of Neuroscience, 2006, 26, pp.1531-1538. ⟨10.1523/JNEUROSCI.4636-05.2006⟩ |
| ISSN: | 0270-6474 1529-2401 |
| Popis: | Dendritic differentiation involves both regressive and growth events. The mechanisms controlling the regressive events are poorly understood. This study is aimed at determining the role of the nuclear receptor retinoid-related orphan receptor α (RORα) in Purkinje cell (PC) dendritic differentiation in organotypic cultures. As observedin vivo, in these cultures, fusiform PCs with embryonic bipolar shape undergo regression before the outgrowth of the ultimate dendritic tree. We show that lentiviral-mediated hRORα1 overexpression in fusiform PCs leads to a cell-autonomous accelerated progression of dendritic differentiation. In addition, RORα is necessary for the PC regressive events: whereasstaggererRORα-deficient PCs remain in the embryonic fusiform stage, replacement of hRORα1 restores normal dendritogenesis. These results demonstrate that RORα expression in fusiform PCs is crucial for the dendritic regression and progression of the following step of extension of dendritic processes. However, it does not seem to participate to the last stage of dendritic growth. This study identifies RORα as a nuclear receptor crucial for the control of dendritic remodeling during development. |
| Databáze: | OpenAIRE |
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