Rhox13 is required for a quantitatively normal first wave of spermatogenesis in mice
| Autor: | Bryan A. Niedenberger, Ellen K. Velte, Nicholas D. Serra, Jonathan T. Busada, Kenneth K Cook, Eugenia H. Goulding, William D. Willis, Edward M. Eddy, Christopher B. Geyer |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Embryology Cellular differentiation Retinoic acid Apoptosis Biology Article Andrology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Spermatocytes medicine Animals Spermatogenesis Cells Cultured Gene knockout Cell Proliferation Epididymis Homeodomain Proteins Mice Knockout Spermatogenic Cell 030219 obstetrics & reproductive medicine Cell growth Obstetrics and Gynecology Cell Differentiation Cell Biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Reproductive Medicine chemistry Fertilization Immunology Female Germ cell |
| Zdroj: | Reproduction. 152:379-388 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-16-0268 |
| Popis: | We previously described a novel germ cell-specific X-linkedreproductivehomeoboxgene (Rhox13) that is upregulated at the level of translation in response to retinoic acid (RA) in differentiating spermatogonia and preleptotene spermatocytes. We hypothesize that RHOX13 plays an essential role in male germ cell differentiation, and have tested this by creating aRhox13gene knockout (KO) mouse.Rhox13KO mice are born in expected Mendelian ratios, and adults have slightly reduced testis weights, yet a full complement of spermatogenic cell types. Young KO mice (at ~7–8 weeks of age) have a ≈50% reduction in epididymal sperm counts, but numbers increased to WT levels as the mice reach ~17 weeks of age. Histological analysis of testes from juvenile KO mice reveals a number of defects during the first wave of spermatogenesis. These include increased apoptosis, delayed appearance of round spermatids and disruption of the precise stage-specific association of germ cells within the seminiferous tubules. Breeding studies reveal that both young and aged KO males produce normal-sized litters. Taken together, our results indicate that RHOX13 is not essential for mouse fertility in a controlled laboratory setting, but that it is required for optimal development of differentiating germ cells and progression of the first wave of spermatogenesis. |
| Databáze: | OpenAIRE |
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