Somatic coliphages are conservative indicators of SARS-CoV-2 inactivation during heat and alkaline pH treatments

Autor: Cédric Hartard, Hélène Jeulin, Raphaël E. Duval, J.-F. Loret, Isabelle Bertrand, Stéphanie Philippot, C. Retourney, Mihayl Varbanov, Christophe Gantzer, M. Gardette, Evelyne Schvoerer
Přispěvatelé: Laboratoire Lorrain de Chimie Moléculaire (L2CM), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Service de Virologie [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Centre International de Recherche Sur l'Eau et l'Environnement [Suez] (CIRSEE), SUEZ ENVIRONNEMENT (FRANCE)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
2019-20 coronavirus outbreak
Veterinary medicine
Environmental Engineering
Hot Temperature
Coronavirus disease 2019 (COVID-19)
Somatic cell
PH
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
viruses
010501 environmental sciences
01 natural sciences
Coliphages
Virus
Article
03 medical and health sciences
Environmental Chemistry
Humans
Somatic coliphages
skin and connective tissue diseases
Waste Management and Disposal
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0105 earth and related environmental sciences
0303 health sciences
Chemistry
SARS-CoV-2
pH
fungi
virus diseases
COVID-19
Hydrogen-Ion Concentration
Pollution
Heat
6. Clean water
3. Good health
body regions
Wastewater
Infectivity
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Sludge
Zdroj: The Science of the Total Environment
Science of the Total Environment
Science of the Total Environment, Elsevier, 2021, 797, pp.149112. ⟨10.1016/j.scitotenv.2021.149112⟩
Science Of The Total Environment (0048-9697) (Elsevier BV), 2021-11, Vol. 797, P. 149112 (8p.)
ISSN: 1879-1026
0048-9697
DOI: 10.1016/j.scitotenv.2021.149112⟩
Popis: High concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome have been described in wastewater and sewage sludge. It raises the question of the security of land sludge disposal practices during a pandemic. This study aimed to compare SARS-CoV-2's resistance to the main inactivating factors in sludge treatments, pH and heat, to that of native wastewater somatic coliphages. The latest can be easily used as an indicator of treatment efficiency in the field. The effects of heat treatment and pH on the survival of SARS-CoV-2 and somatic coliphages were investigated in simple media. The T90 value (time required for a 90% reduction in the virus or a 1 × log10 decline) at 50 °C was about 4 min for infectious SARS-CoV-2, and around 133 min for infectious somatic coliphages, with no decrease in SARS-CoV-2 genome. For infectious SARS-CoV-2, a slight decrease (4 log10) can be considered efficient treatments for infectious SARS-CoV-2. We concluded that somatic coliphages can be considered highly conservative and easy to use indicators of the inactivation of SARS-CoV-2 during treatments based on heat and alkaline pH.
Graphical abstract Unlabelled Image
Databáze: OpenAIRE
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