Shear stress increases hydraulic conductivity of cultured endothelial monolayers
| Autor: | John M. Tarbell, J. R. Artman, Theodore M. Hollis, Yong S. Chang, Howard W. Sill, John A. Frangos |
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| Rok vydání: | 1995 |
| Předmět: |
Endothelium
Physiology Mineralogy Pressure differential Models Biological Hydraulic conductivity 1-Methyl-3-isobutylxanthine Physiology (medical) Monolayer medicine Shear stress Animals Phosphodiesterase inhibitor Cells Cultured Chemistry Thrombin Water Adenosine Endothelial stem cell medicine.anatomical_structure Bucladesine Biophysics Cattle Endothelium Vascular Stress Mechanical Cardiology and Cardiovascular Medicine medicine.drug |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 268:H535-H543 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1995.268.2.h535 |
| Popis: | To examine the effect of shear stress on hydraulic conductivity (Lp) of bovine aortic endothelial cell monolayers grown on polycarbonate filters, we developed a rotating disk system, which imposed a defined shear stress while Lp was measured. A 10-cmH2O pressure differential was applied to monolayers, and baseline Lp was established between 1.65 +/- 0.85 and 4.94 +/- 1.05 x 10(-7) cm.s-1.cmH2O-1. One-hour exposure to 10 dyn/cm2 shear stress caused a significant (P < 0.05) increase in Lp by 2.16-fold (+/- 0.42), and Lp remained elevated when shear stress was removed. Three-hour exposure to shear stresses between 0.1 and 20.0 dyn/cm2 revealed a threshold for shear-induced increase in Lp of 0.5 dyn/cm2. At 20 dyn/cm2, Lp initially decreased by 30% (+/- 13.4%, P < 0.05) and then increased to a level 3.76-fold (+/- 0.83, P < 0.05) greater than baseline Lp at 3 h. The shear-induced increase in Lp was reversed with dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP, 1 mM) and could be significantly (P < 0.05) inhibited when monolayers were preincubated with 0.3 mM DBcAMP, a concentration that did not significantly affect baseline Lp. Furthermore, preincubation with a general phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (1 mM), completely blocked the shear-induced increase in Lp. On the basis of these results, we conclude that shear stress alters endothelial Lp through a cellular mechanism involving signal transduction, not by a purely physical mechanism. |
| Databáze: | OpenAIRE |
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