Plasma fibrinogen inhibits platelets adhesion in flowing blood to immobilized fibrinogen
| Autor: | P. G. De Groot, S. C. Endenburg, J. J. Sixma, J J Zwaginga, L. Lindeboom-Blokzijl |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Chemistry Osmolar Concentration Fibrinogen Adhesion medicine.disease Afibrinogenemia Congenital afibrinogenemia Endocrinology Platelet Adhesiveness Biochemistry Regional Blood Flow Internal medicine medicine Humans Platelet Platelet activation Cardiology and Cardiovascular Medicine Glycoprotein IIb/IIIa IC50 Perfusion medicine.drug |
| Zdroj: | Arteriosclerosis, thrombosis, and vascular biology. 16(5) |
| ISSN: | 1079-5642 |
| Popis: | Abstract The influence of variations in plasma fibrinogen concentration on platelet adhesion to immobilized fibrinogen was investigated in a parallel-plate perfusion chamber. At a shear rate of 1600 s −1 platelet adhesion decreased when increasing concentrations of purified fibrinogen were added to the plasma (IC 50 =1.5±0.2 g/L fibrinogen, n=24). Washed platelets reconstituted in a human albumin solution with red blood cells were more sensitive for soluble fibrinogen (IC 50 =0.4±0.1 g/L, n=5, P 50 of 2.0±0.2 g/L was found (n=9). To exclude the possibility that the inhibition of fibrinogen was caused by irreversible changes in the fibrinogen molecule during the purification procedure, normal plasma was mixed in different ratios with plasma from a patient with congenital afibrinogenemia. Under these conditions, the plasma fibrinogen IC 50 was 1.5±1.1 g/L. Absence of endogenous fibrinogen in the platelets of the patient resulted in an IC 50 of 1.2±0.5 g/L for plasma fibrinogen. These results demonstrate that increased plasma fibrinogen concentrations inhibit platelet adhesion to fibrinogen under flow. |
| Databáze: | OpenAIRE |
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