Differential β3Integrin Expression Regulates the Response of Human Lung and Cardiac Fibroblasts to Extracellular Matrix and Its Components
| Autor: | Cristi R. King, Karen L. Christman, Nick Joseph Merna, Kelsey Michiko Fung, Steven C. George, Kirk C. Hansen, Jean J. Wang |
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| Rok vydání: | 2015 |
| Předmět: |
Swine
Biomedical Engineering Bioengineering Biochemistry Biomaterials Extracellular matrix Tissue engineering medicine Animals Humans Myofibroblasts Fibroblast Lung Cells Cultured Regulation of gene expression Decellularization biology Chemistry Myocardium Integrin beta3 Original Articles Phenotype Actins Extracellular Matrix Cell biology Fibronectin medicine.anatomical_structure Gene Expression Regulation Immunology biology.protein Female Function (biology) |
| Zdroj: | Tissue Engineering Part A. 21:2195-2205 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2014.0337 |
| Popis: | Extracellular matrix (ECM) derived from whole organ decellularization has been successfully used in a variety of tissue engineering applications. ECM contains a complex mixture of functional and structural molecules that are ideally suited for the tissue from which the ECM is harvested. However, decellularization disrupts the structural properties and protein composition of the ECM, which may impact function when cells such as the fibroblast are reintroduced during recellularization. We hypothesized that the ECM structure and composition, fibroblast source, and integrin expression would influence the fibroblast phenotype. Human cardiac fibroblasts (HCFs) and normal human lung fibroblasts (NHLFs) were cultured on intact cardiac ECM, collagen gels, and coatings composed of cardiac ECM, lung ECM, and individual ECM components (collagen and fibronectin [FN]) for 48 h. COL1A expression of HCFs and NHLFs cultured on ECM and FN coatings decreased to |
| Databáze: | OpenAIRE |
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