A flow-through tissue culture system with fast dynamic response
| Autor: | Herbert M. Pinedo, Jan Lankelma, E. Laurensse |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Cell Membrane Permeability
Naphthacenes Biophysics Analytical chemistry Models Biological Biochemistry Cell Line law.invention Mice chemistry.chemical_compound Tissue culture Optical microscope law Culture Techniques Monolayer Animals Melanoma Uridine Molecular Biology Antibiotics Antineoplastic Uracil Neoplasms Experimental Cell Biology Fluorescence chemistry B16 melanoma |
| Zdroj: | Analytical Biochemistry. 127:340-345 |
| ISSN: | 0003-2697 |
| Popis: | A flow-through system in which monolayer cells, growing on a 50-cm 2 glass surface, are in contact with a film of medium with a thickness of 0.14 mm, is described. For murine B16 melanoma cells, the loading capacity is 8.10 6 cells. The flow-through principle permits frequent off-line or on-line detection of medium constituents for a period on the order of days. The system has a fast dynamic response. With off-line radiochemical detection, the system was applied to the uptake of uridine and excretion of uracil over a period of 45 h. With on-line fluorescence detection, the interaction between the cells and two anthracycline analogs was monitored. The cells can be easily observed with a light microscope. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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