Study on the diversity of Bacteroides and Clostridium in patients with primary gout
| Autor: | Xi-Shuang Liu, Shi-Chao Xing, Dong-Mei Meng, Changgui Li, Na Li, Jiang Gang, Yao-Yao Yan, Ying Chen |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
musculoskeletal diseases
Adult Male congenital hereditary and neonatal diseases and abnormalities Gout Biophysics Gut flora Biochemistry Polymerase Chain Reaction law.invention Microbiology Clostridium law RNA Ribosomal 16S medicine Bacteroides Cluster Analysis Humans Feces Polymerase chain reaction biology nutritional and metabolic diseases food and beverages Cell Biology General Medicine Biodiversity Ribosomal RNA Middle Aged biology.organism_classification medicine.disease 16S ribosomal RNA Case-Control Studies |
| Zdroj: | Cell biochemistry and biophysics. 71(2) |
| ISSN: | 1559-0283 |
| Popis: | To analyze the diversity of both Bacteroides and Clostridium in patients with primary gout and the difference from that of normal individuals. And to investigate the relationship between the primary gout and the intestinal flora. Fecal samples of 90 cases with the primary gout and 94 cases normal comparison group were selected, together with the cases that match the filter criteria. The DNA is extracted from the feces. 16S rRNA specific primers of both Bacteroides and Clostridium were adopted for the PCR amplification. The molecular fingerprints of Bacteroides and Clostridium in both the primary gout group and the normal control group were obtained through DGGE and subjected for further analysis on both the diversity and the similarity. Compared with normal individuals, the number of bands and Shannon-Weaver (H') of Bacteroides in patients with primary gout was not reduced, but significantly decreased in Clostridium. Furthermore, the intra-group and inter-group similarity of both Bacteroides and Clostridium were lower. The primary gout has caused the structural change of both Bacteroides and Clostridium, inducing the low similarity, especially for Clostridium. It has statistic significance. The gut predominant flora may play an important role in the development of primary gout. |
| Databáze: | OpenAIRE |
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