Formation of C19 11-hydroxysteroids by porcine Leydig cells
| Autor: | R. L. Renaud, M. W. Khalil, J.I. Raeside |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Hydrocortisone Swine Testicular vein Cell Steroid biosynthesis Biology Biochemistry High-performance liquid chromatography Internal medicine medicine Animals Androstenedione Molecular Biology Cells Cultured Chromatography High Pressure Liquid Hydroxysteroids Leydig Cells Substrate (chemistry) Cell Biology Endocrinology medicine.anatomical_structure medicine.vein Gas chromatography |
| Zdroj: | Biochemistry and Cell Biology. 70:174-176 |
| ISSN: | 1208-6002 0829-8211 |
| DOI: | 10.1139/o92-026 |
| Popis: | In a previous study, we reported the presence of 11β-hydroxyandrostenedione and 11β-hydroxytestosterone in testicular vein blood from mature male pigs. Since C19 steroids with an oxygen function at C11 have not been recorded as products of steroid biosynthesis in normal mammalian testes, we have examined their possible production in purified preparations of porcine Leydig cells. Both androstenedione and cortisol were added as substrates in studies using cell incubations of Leydig cells from mature boars (> 8 months old). Steroids were recovered from media by solid-phase extraction and separated by reversed-phase high performance liquid chromatography. Peaks corresponding to retention times of authentic standard steroids were seen for both 11β-hydroxyandrostenedione and 11β-hydroxytestosterone from each substrate. Generally, lesser amounts of C19 11-oxosteroids were noted also. Definitive confirmation was made by gas chromatography – mass spectrometry for 11β-hydroxyandrostenedione in the media.Key words: 11β-hydroxylation, androgens, Leydig cells, porcine, testis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|