Reduction of Cav1.3 channels in dorsal hippocampus impairs the development of dentate gyrus newborn neurons and hippocampal-dependent memory tasks
| Autor: | Ye Ryoung Park, Byung Il Choi, Bo-Young Lee, Hyun Kim, Chong Hyun Kim, Su Hyun Kim |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male lcsh:Medicine Hippocampus Social Sciences Hippocampal formation Cav1.3 Mice 0302 clinical medicine Cognition Learning and Memory Animal Cells Hippocampal Neurogenesis Medicine and Health Sciences Psychology Pseudopodia RNA Small Interfering lcsh:Science Mice Knockout Neurons Cognitive Impairment Multidisciplinary Microscopy Confocal biology Voltage-dependent calcium channel Behavior Animal Cognitive Neurology Neurogenesis Brain Fear Dependovirus Neurology RNA Interference Cellular Types Anatomy Filopodia Research Article Mossy fiber (hippocampus) Memory Long-Term Calcium Channels L-Type Cognitive Neuroscience Genetic Vectors 03 medical and health sciences Developmental Neuroscience Memory Animals Learning Dentate gyrus lcsh:R Cognitive Psychology Biology and Life Sciences Dendrites Cell Biology Neuronal Dendrites Mice Inbred C57BL 030104 developmental biology Retroviridae Cellular Neuroscience Dentate Gyrus biology.protein Cognitive Science lcsh:Q Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 7, p e0181138 (2017) |
| ISSN: | 1932-6203 |
| Popis: | Cav1.3 has been suggested to mediate hippocampal neurogenesis of adult mice and contribute to hippocampal-dependent learning and memory processes. However, the mechanism of Cav1.3 contribution in these processes is unclear. Here, roles of Cav1.3 of mouse dorsal hippocampus during newborn cell development were examined. We find that knock-out (KO) of Cav1.3 resulted in the reduction of survival of newborn neurons at 28 days old after mitosis. The retroviral eGFP expression showed that both dendritic complexity and the number and length of mossy fiber bouton (MFB) filopodia of newborn neurons at ≥ 14 days old were significantly reduced in KO mice. Both contextual fear conditioning (CFC) and object-location recognition tasks were impaired in recent (1 day) memory test while passive avoidance task was impaired only in remote (≥ 20 days) memory in KO mice. Results using adeno-associated virus (AAV)-mediated Cav1.3 knock-down (KD) or retrovirus-mediated KD in dorsal hippocampal DG area showed that the recent memory of CFC was impaired in both KD mice but the remote memory was impaired only in AAV KD mice, suggesting that Cav1.3 of mature neurons play important roles in both recent and remote CFC memory while Cav1.3 in newborn neurons is selectively involved in the recent CFC memory process. Meanwhile, AAV KD of Cav1.3 in ventral hippocampal area has no effect on the recent CFC memory. In conclusion, the results suggest that Cav1.3 in newborn neurons of dorsal hippocampus is involved in the survival of newborn neurons while mediating developments of dendritic and axonal processes of newborn cells and plays a role in the memory process differentially depending on the stage of maturation and the type of learning task. |
| Databáze: | OpenAIRE |
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