Gene expression profiles in the dorsal root ganglia of methylmercury-exposed rats
| Autor: | Toshiyuki Kaji, Yasuyuki Fujiwara, Eiko Yoshida, Yo Shinoda, Satoshi Tatsumi, Tsutomu Takahashi, Komyo Eto |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Central nervous system Administration Oral 010501 environmental sciences Biology Toxicology 030226 pharmacology & pharmacy 01 natural sciences 03 medical and health sciences 0302 clinical medicine Dorsal root ganglion Ganglia Spinal Gene expression medicine Animals KEGG Rats Wistar Gene 0105 earth and related environmental sciences Oligonucleotide Array Sequence Analysis Inflammation Neurotoxicity Methylmercury Compounds medicine.disease Molecular biology medicine.anatomical_structure Peripheral nervous system DNA microarray Transcriptome |
| Zdroj: | The Journal of toxicological sciences. 44(8) |
| ISSN: | 1880-3989 |
| Popis: | Methylmercury (MeHg) exposure is known to induce neurodegeneration in both the central nervous system (CNS) and peripheral nervous system (PNS). Molecular mechanisms of MeHg-induced neurotoxicity have been well investigated in the CNS, however, it remains unclear in the PNS. In the present study, comprehensive gene expression analysis was performed by analyzing MeHg-exposed adult rat dorsal root ganglion (DRG) by DNA microarray. Methylmercuric chloride (6.7 mg/kg/day) was administered to nine-week-old male Wistar rats for five days, followed by two days without administration; this cycle was repeated once. Rats were anesthetized at 7 or 14 days after commencement of MeHg exposure, and their DRGs were removed and homogenized to make total RNA samples. DNA microarray data from Day 7 samples identified 100 out of 18,513 detected genes as annotated genes with more than two-fold upregulated or downregulated expression compared with controls. Database for Annotation, Visualization, and Integrated Discovery (DAVID) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses suggested strong involvement of immune activation and inflammation pathways in rat DRG exposed to MeHg, and some genes overlapped with previously reported genes affected by MeHg exposure in the cerebellum. The present results suggest that MeHg-induced neurotoxicity is associated with immune activation and inflammatory responses in rat DRG. |
| Databáze: | OpenAIRE |
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