Insulin Secretion Studied in the Perfused Rat Pancreas: II. Effect of Glucose, Glucagon, 3'5' Adenosine Monophosphate, Theophylline, Imidazole and Phenoxybenzamine; Their Interaction with Diazoxide
| Autor: | J C Basabe, N L Lopez, J K Viktora, F W Wolff, B McNeal, C Mongelli, K WcInhardt, A Baukal |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Male
Adenosine monophosphate medicine.medical_specialty Phenoxybenzamine Endocrinology Diabetes and Metabolism medicine.medical_treatment Radioimmunoassay Buffers Glucagon chemistry.chemical_compound Theophylline Internal medicine Insulin Secretion Cyclic AMP Internal Medicine medicine Diazoxide Animals Insulin Cyclic adenosine monophosphate Pancreas Adrenergic alpha-Antagonists Adenine Nucleotides Imidazoles Stimulation Chemical Rats Perfusion Glucose Endocrinology chemistry Basal (medicine) medicine.drug |
| Zdroj: | Diabetes. 20:457-466 |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/diab.20.7.457 |
| Popis: | This communication reports studies dealing with the action of glucagon, theophylline, cyclic adenosine monophosphate (3′5′.AMP) and phenoxybenzamine on insulin secretion, and their possible interaction with glucose and diazoxide. Diazoxide inhibited both phases of insulin secretion produced by glucose. This blocking action was suppressed when the alpha-blocking phenoxybenzamine was present. Glucagon produced a uniform pattern of insulin secretion when perfused alone. A one-minute pulse of 3′5′.AMP produced a sharp peak of insulin secretion. Neither glucagon nor 3′5′.AMP effects were inhibited by diazoxide. Theophylline did not increase the basal insulin-secretion. However, when perfused together with glucose, theophylline stimulated additional insulin secretion, significantly higher than that produced by glucose alone. When combinations of glucagon plus glucose, 3′5′.AMP plus glucose and theophylline plus glucose, were perfused, diazoxide blocked the secretion of insulin stimulated by glucose, but not that produced by glucagon, 3′5′.AMP or theophylline. This suggests that glucagon, 3′5′.AMP and theophylline stimulate insulin secretion by actingon mechanismsdifferent from the ones stimulated by glucose |
| Databáze: | OpenAIRE |
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