Tissue engineering of diseased bladder using a collagen scaffold in a bladder exstrophy model

Autor:	Toin H. van Kuppevelt, L.A.J. Roelofs, Alex J. Eggink, Paul J. Geutjes, Dorien M. Tiemessen, Egbert Oosterwijk, Willeke F. Daamen, Barbara B.M. Kortmann, Wout F.J. Feitz, A. Jane Crevels, Rene M. H. Wijnen
Přispěvatelé:	Obstetrics & Gynecology, Pediatric Surgery
Rok vydání:	2014
Předmět:	Pathology medicine.medical_specialty Fetus Urinary bladder business.industry Urology Regeneration (biology) Rare cancers Radboud Institute for Molecular Life Sciences [Radboudumc 9] medicine.disease Extracellular matrix Bladder exstrophy medicine.anatomical_structure Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] Tissue engineering Submucosa Urological cancers Radboud Institute for Molecular Life Sciences [Radboudumc 15] medicine business Survival rate
Zdroj:	BJU International, 114, 3, pp. 447-57 BJU International, 114, 447-57 BJU International, 114(3), 447-457. Wiley-Blackwell Publishing Ltd
ISSN:	1464-410X 1464-4096
DOI:	10.1111/bju.12591
Popis:	Item does not contain fulltext OBJECTIVE: To compare the regenerative capacity of diseased bladder in a large animal model of bladder exstrophy with regeneration in healthy bladder using a highly porous collagen scaffold. MATERIALS AND METHODS: Highly porous bovine type I collagen scaffolds with a diameter of 32 mm were prepared. In 12 fetal sheep a bladder exstrophy was surgically created at 79 days' gestation. Lambs were born at full term (140 days' gestation). After 1 week the bladder lesion was reconstructed and augmented with a collagen scaffold (group 1). In nine normal newborn lambs the bladder was augmented with a collagen scaffold 1 week after birth (group 2). Functional (video-urodynamics) and histological evaluation was performed at 1 and 6 months after surgery. RESULTS: The survival rate was 58% in group 1 and 100% in group 2. Cystograms were normal in all lambs, besides low-grade reflux in both groups. Urodynamics showed comparable capacity between both groups and a trend to lower compliance in group 1. Histological evaluation at 1 month revealed a non-confluent urothelial layer, an immature submucosa, and initial ingrowth of smooth muscle cells. At 6 months both groups showed normal urothelial lining, standard extracellular matrix development, and smooth muscle cell ingrowth. CONCLUSIONS: Bladder tissue regeneration with a collagen scaffold in a diseased bladder model and in healthy bladder resulted in comparable functional and histological outcome, with a good quality of regenerated tissue involving all tissue layers. Improvements may still be needed for larger augmentations or more severely diseased bladders.
Databáze:	OpenAIRE
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