Two-Phase Biocatalysis in Microfluidic Droplets
| Autor: | Felix Kaspar, Iordania Constantinou, Anett Schallmey, Lanting Xiang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Mass transport
Microfluidics Clinical Biochemistry microfluidics Nanotechnology two-phase biocatalysis Article Phase (matter) Two-phase Biocatalysis ddc:6 Veröffentlichung der TU Braunschweig ddc:62 ddc:5 microfluidic droplets Chemistry Microfluidic Droplets Water General Medicine Organic media ddc:57 enzyme Enzyme Biocatalysis Perspective Publikationsfonds der TU Braunschweig ddc:600 TP248.13-248.65 Biotechnology |
| Zdroj: | Biosensors, Vol 11, Iss 407, p 407 (2021) Biosensors 2021, 11(11), 407--Biosensors (Basel)--http://www.mdpi.com/journal/biosensors--https://www.ncbi.nlm.nih.gov/pmc/journals/2620/--http://www.bibliothek.uni-regensburg.de/ezeit/?2662125--2079-6374--2079-6374 Biosensors |
| ISSN: | 2079-6374 |
| Popis: | This Perspective discusses the literature related to two-phase biocatalysis in microfluidic droplets. Enzymes used as catalysts in biocatalysis are generally less stable in organic media than in their native aqueous environments; however, chemical and pharmaceutical compounds are often insoluble in water. The use of aqueous/organic two-phase media provides a solution to this problem and has therefore become standard practice for multiple biotransformations. In batch, two-phase biocatalysis is limited by mass transport, a limitation that can be overcome with the use of microfluidic systems. Although, two-phase biocatalysis in laminar flow systems has been extensively studied, microfluidic droplets have been primarily used for enzyme screening. In this Perspective, we summarize the limited published work on two-phase biocatalysis in microfluidic droplets and discuss the limitations, challenges, and future perspectives of this technology. |
| Databáze: | OpenAIRE |
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