Audiogenic seizure susceptibility is reduced in fragile X knockout mice after introduction of FMR1 transgenes
| Autor: | Simona D'Antoni, Cathy E. Bakker, Sebastiano A. Musumeci, Giuseppe Calabrese, Ben A. Oostra, Maria Vincenza Catania, David L. Nelson, Raffaele Ferri, Judith R. Brouwer, Maurizio Elia, Carmela M. Bonaccorso |
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| Přispěvatelé: | Clinical Genetics |
| Rok vydání: | 2007 |
| Předmět: |
Genetically modified mouse
congenital hereditary and neonatal diseases and abnormalities Aging endocrine system diseases Ratón Transgene Biology Transfection Epilepsy Reflex Epilepsy Fragile X Mental Retardation Protein Mice Developmental Neuroscience medicine Animals Humans Genetic Predisposition to Disease Transgenes Mice Knockout transgenics Wild type Age Factors Brain Genetic Therapy medicine.disease FMR1 Phenotype Molecular biology nervous system diseases Disease Models Animal Neurology Acoustic Stimulation Fragile X Syndrome Immunology Knockout mouse FRAX |
| Zdroj: | Experimental Neurology, 203(1), 233-240. Academic Press Experimental neurology 203 (2007): 233–240. doi:10.1016/j.expneurol.2006.08.007 info:cnr-pdr/source/autori:Musumeci SA, Calabrese G, Bonaccorso CM, D'Antoni S, Brouwer JR, Bakker CE, Elia M, Ferri R, Nelson DL, Oostra BA, Catania MV./titolo:Audiogenic seizure susceptibility is reduced in fragile X knockout mice after introduction of FMR1 transgenes./doi:10.1016%2Fj.expneurol.2006.08.007/rivista:Experimental neurology/anno:2007/pagina_da:233/pagina_a:240/intervallo_pagine:233–240/volume:203 |
| ISSN: | 1090-2430 0014-4886 |
| DOI: | 10.1016/j.expneurol.2006.08.007 |
| Popis: | The Fmr1 knockout (KO) mouse is characterized by an increased audiogenic seizure (AGS) susceptibility and is considered a good animal model for epilepsy and seizures in the human fragile-X (FRAX) syndrome. Here, we tested the hypothesis that the reintroduction of the FMR1 gene is able to revert the AGS susceptibility characterizing Fmr1 KO mice. To this aim, two groups of Fmr1 KO transgenic mice, which have additional copies of the human FMR1 gene (YAC) or FMR1 cDNA (G6) were used. AGS susceptibility of these mice was examined and compared to that of Fmr1 KO, wild type, and wild-type animals in whom the FMR1gene was also introduced (over-expressed). Mice were tested at different ages because AGS susceptibility is age dependent. The intensity of response was scored and the results were analyzed by means of 2-way analysis of variance to evaluate the effects of age and genetic condition. We found that AGS susceptibility rescue is complete in the G6 mice and partial in YAC mice. Our data indicate that the introduction of the human FMR1 gene in Fmr1 KO mice is able to revert the Fmr1 KO epileptic phenotype. |
| Databáze: | OpenAIRE |
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