Differentially expressed gene transcripts in wheat and barley leaves upon leaf spot infection
| Autor: | Alena Hanzalová, Veronika Dumalasová, Leona Svobodová |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
0301 basic medicine Appressorium biology Inoculation cDNA library Pyrenophora food and beverages Plant Science Plant disease resistance biology.organism_classification 01 natural sciences 03 medical and health sciences Horticulture Pathosystem 030104 developmental biology Botany Genetics Leaf spot Cultivar 010606 plant biology & botany |
| Zdroj: | ResearcherID |
| ISSN: | 1805-9325 1212-1975 |
| DOI: | 10.17221/187/2011-cjgpb |
| Popis: | A cDNA library was created on the basis of transcripts that were generated during the process of infection of wheat and barley with Pyrenophora tritici-repentis or P. teres . Due to the time course of infection assays, the leaves were collected at various intervals after inoculation until 100 h after inoculation. We compared the temporal development of the two pathogens, as well as the effect of varieties of the same crop species, and differences among isolates of a single pathogen. The appressoria and vesicular structures developed more rap - idly in barley varieties inoculated by P. teres than in wheat varieties inoculated by P. tritici-repentis . The lower level of susceptibility of the wheat variety Globus and the barley variety Heris to P. tritici-repentis and P. teres, respectively, was apparent from 12 h after infection. Information on changes in expression profiles during pathogenesis and on the clones that could be functionally assigned to the known gene sequences is presented. Out of the 360 transcript derived fragments (TDFs) produced in the barley- P. teres pathosystem in our trials, 62% were specific to the plant-pathogen interaction. In the wheat- P. tritici-repentis pathosystem 291 TDFs oc |
| Databáze: | OpenAIRE |
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