Analysis of the immunoglobulin heavy chain gene of secondary diffuse large B-cell lymphoma that subsequently developed in four cases with B-cell chronic lymphocytic leukemia or lymphoplasmacytoid lymphoma (Richter syndrome)
| Autor: | Masafumi Abe, Kunihiko Maeda, Kunihiko Tominaga, Michiko Sato, Yuko Hashimoto, Sayuri Hoshi, Tetsuo Kuze, Asumi Shirakawa, Naoya Nakamura, Yoshikazu Sasaki |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Lymphoma
B-Cell Chronic lymphocytic leukemia Molecular Sequence Data Clone (cell biology) chemical and pharmacologic phenomena Biology CD5 Antigens Polymerase Chain Reaction Pathology and Forensic Medicine Germline mutation Antigen immune system diseases hemic and lymphatic diseases medicine Humans neoplasms Aged DNA Primers Aged 80 and over Base Sequence Neoplasms Second Primary hemic and immune systems General Medicine Middle Aged medicine.disease Immunohistochemistry Leukemia Lymphocytic Chronic B-Cell Clone Cells Neoplasm Proteins Lymphoma Cancer research Immunoglobulin heavy chain Lymphoma Large B-Cell Diffuse CD5 Immunoglobulin Heavy Chains Diffuse large B-cell lymphoma |
| Zdroj: | Pathology International. 50:636-643 |
| ISSN: | 1440-1827 1320-5463 |
| Popis: | The immunoglobulin heavy chain gene (IgH gene) was analysed in four cases of B-cell Richter syndrome, in order to determine whether a secondary diffuse large B-cell lymphoma (DLBCL) could arise from the same clone as the initial B-cell chronic lymphocytic leukemia (B-CLL) and lymphoplasmacytoid lymphoma (LPL) or be a de novo event, and whether secondary DLBCL shows an intraclonal microheterogeneity. Both the initial B-CLL and secondary DLBCL in two cases expressed CD5 antigen. Both samples of the initial B-CLL or LPL and the secondary DLBCL in three cases were examined for comparison. The polymerase chain reaction-amplified IgH gene of secondary DLBCL in two cases (CD5+ case and CD5- case) were different from those of the initial B-CLL, revealing a new malignant clone. The other case (CD5-) showed that secondary DLBCL had a sequence identical to the initial LPL, indicating the same clonal origin. The variable region of the IgH gene of secondary DLBCL (CD5+ two cases and CD5- two cases) exhibited a 0.5-9.0% somatic mutation range and no intraclonal microheterogeneity. |
| Databáze: | OpenAIRE |
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