Full characterization of three toxins from the Androctonus amoreuxi scorpion venom

Autor: Christian Legros, Brigitte Ceard, Marie-France Martin-Eauclaire, Maya Belghazi, Alain Hamon, Najwa Abbas, Pierre E. Bougis
Přispěvatelé: Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), Récepteurs et Canaux Ioniques Membranaires (RCIM)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Patch-Clamp Techniques
MESH: Drosophila
Xenopus
Scorpion Venoms
Venom
MESH: Amino Acid Sequence
Chemical Fractionation
Toxicology
medicine.disease_cause
Inbred C57BL
Median lethal dose
Sodium Channels
Mice
MESH: Scorpion Venoms
Drosophila Proteins
MESH: Immune Sera
MESH: Animals
MESH: Xenopus
0303 health sciences
biology
MESH: Kinetics
030302 biochemistry & molecular biology
MESH: Enzyme-Linked Immunosorbent Assay
Anatomy
3. Good health
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
Biochemistry
Drosophila
Sodium Channel Blockers
MESH: Rats
MESH: Drosophila Proteins
Molecular Sequence Data
Scorpion
MESH: Sequence Alignment
Enzyme-Linked Immunosorbent Assay
MESH: Sodium Channels
Lethal Dose 50
Scorpions
03 medical and health sciences
MESH: Chemical Fractionation
MESH: Mice
Inbred C57BL
biology.animal
MESH: Patch-Clamp Techniques
medicine
Animals
Amino Acid Sequence
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
MESH: Mice
030304 developmental biology
Antiserum
MESH: Molecular Sequence Data
Toxin
Sodium channel
Immune Sera
biology.organism_classification
MESH: Scorpions
Rats
Mice
Inbred C57BL
Kinetics
MESH: Lethal Dose 50
MESH: Sodium Channel Blockers
Buthus occitanus
Sequence Alignment
Zdroj: Toxicon
Toxicon, Elsevier, 2009, 54 (4), pp.460-70. ⟨10.1016/j.toxicon.2009.05.020⟩
Toxicon, 2009, 54 (4), pp.460-70. ⟨10.1016/j.toxicon.2009.05.020⟩
ISSN: 0041-0101
DOI: 10.1016/j.toxicon.2009.05.020⟩
Popis: International audience; In this study, we have characterized the immunological and pharmacological properties of the three major alpha-type toxins from the scorpion Androctonus amoreuxi, AamH1, AamH2 and AamH3, which were previously described as putative toxins from cDNAs [Chen, T. et al., 2003. Regul. Pept. 115, 115-121]. The immunological tests (ELISA, RIA) have demonstrated that AamH1, AamH2 and AamH3 belong to the immunological groups 3 and 4 of alpha-type toxins. Analysis of the three toxin effects on currents through rat brain (rNav1.2), rat muscle (rNav1.4) and Drosophila (DmNav1) sodium channels expressed in Xenopus oocytes revealed that AamH1 and AamH2, but not AamH3, have anti-insect and anti-mammal activities and can be classified as alpha-like toxins. While AamH1 removes fast inactivation only in neuronal rNav1.2 channel and has no effect on muscular rNav1.4 channel, AamH2 affects both neuronal rNav1.2 and muscular rNav1.4 channels. AamH3 was lethal to mice by intracerebroventricular injection despite its lack of activity on the neuronal rNav1.2 channel. Finally, we have shown that the A. amoreuxi venom was better neutralized by the antiserum raised against the venom of Buthus occitanus tunetanus than by the antisera raised against scorpion venoms from the same genus Androctonus.
Databáze: OpenAIRE
Externí odkaz: