Cloned Viral Protein Vaccine for Foot-and-Mouth Disease: Responses in Cattle and Swine
| Autor: | Douglas M. Moore, D. O. Morgan, Daniel G. Yansura, Marvin J. Grubman, P. D. McKercher, Donald Dowbenko, Howard L. Bachrach, Dennis G. Kleid, B.H. Robertson, Barbara Small |
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| Rok vydání: | 1981 |
| Předmět: |
Transcription
Genetic Swine Viral protein viruses DNA Recombinant Cattle Diseases Biology medicine.disease_cause Virus Viral Proteins Plasmid medicine Animals Amino Acid Sequence Cloning Molecular Neutralizing antibody Escherichia coli Swine Diseases Immunity Cellular Vaccines Multidisciplinary Base Sequence RNA DNA Restriction Enzymes Fusion protein Virology Capsid Foot-and-Mouth Disease Protein Biosynthesis Antibody Formation biology.protein Cattle |
| Zdroj: | Science. 214:1125-1129 |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | A DNA sequence coding for the immunogenic capsid protein VP3 of foot-and-mouth disease virus A12, prepared from the virion RNA, was ligated to a plasmid designed to express a chimeric protein from the Escherichia coli tryptophan promoter-operator system. When Escherichia coli transformed with this plasmid was grown in tryptophan-depleted media, approximately 17 percent of the total cellular protein was found to be an insoluble and stable chimeric protein. The purified chimeric protein competed equally on a molar basis with VP3 for specific antibodies to foot-and-mouth disease virus. When inoculated into six cattle and two swine, this protein elicited high levels of neutralizing antibody and protection against challenge with foot-and-mouth disease virus. |
| Databáze: | OpenAIRE |
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