Sodium hyaluronate-induced ocular hypertension in rats damages the direction-selective circuit and inner-outer retinal plexiform layers
| Autor: | Agustina Noailles, Oksana Kutsyr, Aloma Mayordomo-Febrer, Pedro Lax, María López-Murcia, Silvia M. Sanz-González, María Dolores Pinazo-Durán, Nicolás Cuenca |
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| Přispěvatelé: | Producción Científica UCH 2022, UCH. Departamento de Medicina y Cirugía Animal, Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología, Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS) |
| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2022 |
| Předmět: |
Male
Retinal Ganglion Cells Morphology genetic structures Eye - Diseases Direction-selective ganglion cells Biología Celular Cells Fisiología Retina Animals Humans Hyaluronic Acid Rats Wistar Glaucoma Intraocular Pressure Retina - Diseases - Treatment Immunohistochemistry eye diseases Rats Disease Models Animal Células Chronic glaucoma model Ojos - Enfermedades Ocular Hypertension sense organs Starburst amacrine cells Glaucoma Open-Angle |
| Zdroj: | RUA. Repositorio Institucional de la Universidad de Alicante Universidad de Alicante (UA) |
| Popis: | Purpose: To assess the changes in retinal morphology in a rat model of chronic glaucoma induced by ocular hypertension. Methods: Intraocular pressure (IOP) was surgically increased through weekly injections of sodium hyaluronate (HYA) in the anterior eye chamber of the left eye of male Wistar rats, whereas the right eyes were sham operated (salt solution). During the 10-week experimental period, IOP was measured weekly with a rebound tonometer. Retinal cryosections were prepared for histological/immunohistochemical analysis and morphometry. Results: IOP was higher in HYA-treated eyes than in sham-operated eyes along the 10-week period, which was significant from the fourth to the nineth week. Ocular hypertension in HYA-treated eyes was associated with morphologic and morphometric changes in bipolar cells, ON-OFF direction-selective ganglion cells, ON/OFF starburst amacrine cells, and inner plexiform layer sublamina. Conclusions: Serial HYA treatment in the rat anterior eye chamber results in mild-to-moderate elevated and sustained IOP and ganglion cell death, which mimics most human open-angle glaucoma hallmarks. The reduced number of direction-selective ganglion cells and starburst amacrine cells accompanied by a deteriorated ON/OFF plexus in this glaucoma model could lend insight to the abnormalities in motion perception observed in patients with glaucoma. Supported by the Spanish Ministry of Science and Innovation (MICINN-FEDER PID2019-106230RB-I00), 2), Institute of Health Carlos III (General Subdirection of Networks and Cooperative Research Centers RETICs OFTARED 2012-2021) co-financed by the European Regional Development fund (RD16/0008/0016 [Alicante] and RD16/0008/0022 [Valencia]), Asociación Retina Asturias (ASOCIACIONRETINA1-20I), Generalitat Valenciana (IDIFEDER/2017/064), and the Research funds from both Department of Surgery (Ophthalmology Unit) and Cellular-Molecular Ophthalmobiology Group of the University of Valencia (Spain). |
| Databáze: | OpenAIRE |
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