Differentiation, apoptosis and GM-CSF receptor expression of human gingival fibroblasts on a titanium surface treated by a dual acid-etched procedure
| Autor: | Salvatore Salzano, Gabriele Capece, Luca Ramaglia, Gaetano Di Spigna, Loredana Postiglione, Carolina Sbordone |
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| Přispěvatelé: | Ramaglia, Luca, Gaetano Di, Spigna, Gabriele, Capece, Sbordone, Carolina, Salvatore, Salzano, Postiglione, Loredana |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Surface Properties
Cell Gingiva Tenascin Dentistry Apoptosis In Vitro Techniques Extracellular matrix Dental Materials stomatognathic system Acid Etching Dental medicine Cell Adhesion Humans Cell adhesion Receptor General Dentistry Cells Cultured Cell Proliferation Titanium Extracellular Matrix Proteins biology Cell growth Chemistry business.industry Cell Differentiation Fibroblasts Molecular biology Fibronectin medicine.anatomical_structure Receptors Granulocyte-Macrophage Colony-Stimulating Factor biology.protein business |
| Popis: | Analysis of the effects of titanium surface properties on the biological behavior of human gingival fibroblasts (HGFs). HGFs were in vitro cultured on a titanium surface modified by a dual acid-etched procedure and on a control machined surface. Cell adhesion, proliferation, apoptosis, production of certain extracellular matrix (ECM) proteins, and expression of granulocyte macrophage-colony stimulating factor receptor (GM-CSFR) were investigated using in each experiment a total of 18 samples for each titanium surface. Cell attachment at 3 h of culture was statistically significantly higher on the etched surface. HGF growth increased on both surfaces during the entire experimental period and at day 14 of culture cell proliferation was statistically significantly higher on the treated surface than on the control. No statistically significant differences in percentage of apoptosis events were observed between the surfaces. ECM protein production increased progressively over time on both surfaces. A statistically significant deposition was observed at day 7 and 14 for collagen I and only at day 14 for fibronectin and tenascin, when compared to the baseline. GM-CSFR registered a positive expression on both surfaces, statistically significant at day 14 on the etched surface in comparison with the machined one. Data showed that titanium surface microtopography modulates in vitro cell response and phenotypical expression of HGFs. The etched surface promoted a higher cell proliferation and differentiation improving the biological behavior of HGFs. Results suggest a possible beneficial effect of surface etching modification on peri-implant biological integration and soft tissue healing which is critical for the formation of a biological seal around the neck of dental implants. |
| Databáze: | OpenAIRE |
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